Pengaruh Modifikasi Sumber Nitrogen Dari Pupuk Calcium Ammonium Nitrate terhadap Pertumbuhan, Biomassa, dan Kandungan Metabolit Pada Kultur Semi-Massal Spirulina sp.

WILDAN HILMI AZHARUL HAKIM, Dr. Eko Agus Suyono, M.App.Sc

2024 | Skripsi | BIOLOGI

Spirulina sp. merupakan salah satu kelompok Cyanobacteria yang mudah ditemukan dan saat ini sedang ramai dimanfaatkan dalam banyak bidang bioteknologi, salah satunya bidang pangan fungsional. Spirulina sp. memiliki kandungan metabolit seperti karbohidrat, lipid, protein, karoten, asam nukleat dan metabolit sekunder lainnya. Kandungan protein pada Spirulina sp. mencapai 60 - 70?ri total berat kering biomassa. Penambahan nitrogen memberikan banyak efek terhadap Spirulina sp. seperti laju pertumbuhan, kandungan biomassa dan jalur metabolisme pada Spirulina sp. Salah satu pupuk komersial yang mengandung sumber nitrogen melimpah berupa NO3- dan NH4+ adalah pupuk CAN (Calcium Ammonium Nitrate). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sumber nitrogen dari pupuk CAN (Calcium Ammonium Nitrate) terhadap laju pertumbuhan, kandungan biomassa, dan metabolit primer pada Spirulina sp. dalam skala kultur semi-massal. Perlakuan pupuk yang ditambahkan pada penelitian ini adalah kontrol (0g/L), C1 (0,71 g/L), C2 (1,1 g/L), dan C3 (1,5 g/L). Penelitian dilakukan secara bertahap, dimulai dari preparasi medium, preparasi perlakuan, Scale-up kultur hingga volume semi-massal, Running penelitian, perhitungan laju pertumbuhan, uji kandungan biomassa dan pengujian metabolit serta pengolahan data. Hasil yang didapatkan pada penelitian ini adalah perlakuan C3 meningkatkan laju pertumbuhan, kandungan protein (39,950 mg/mL ± 2,51b) dan pigmen klorofil-a (2,669 mg/mL ± 1,10b) tertinggi dibandingkan perlakuan lainnya, sedangkan perlakuan C1 meningkatkan kandungan biomassa (1,561 mg/mL ± 0,0586b), lipid (0,850 mg/mL ± 0,02) dan karbohidrat (4,83 mg/L ± 0,22a) tertinggi dibandingkan perlakuan lainnya. Berdasarkan hasil penelitian, penambahan pupuk CAN (Calcium Ammonium Nitrate) meningkatkan laju pertumbuhan dan metabolit primer kultur semi-massal Spirulina sp.

Spirulina sp. is a group of cyanobacteria that is relatively easy to find and is currently being utilised in numerous fields of biotechnology. One such field is the field of functional food. In addition, Spirulina sp. contains a range of metabolites, including carbohydrates, lipids, proteins, carotene, nucleic acids, and other secondary metabolites, with a notable abundance of protein content. The protein content in Spirulina sp. reaches 60 - 70 % of the total dry weight. The addition of nitrogen has a profound effect on the growth rate, biomass content, and metabolic pathways of Spirulina sp. One of the most common commercial fertilizer that contains abundant nitrogen sources in the form of NO3- and NH4+ is CAN (Calcium Ammonium Nitrate) fertilizer. The objective of this study is to determine the impact of nitrogen sources from CAN (Calcium Ammonium Nitrate) fertilizer on growth rate, biomass content and primary metabolites in Spirulina sp. in semi-mass culture scale. The fertilizer treatments employed in this study were control (0 g/L); C1 (0,71 g/L), C2 (1,1 g/L), C3 (1,5 g/L). The research wa conducted  in a series of stages, beginning with the preparation of the medium, the preparation of the treatment, the scale-up culture to semi-mass volume, the running of the research, the calculation of the growth rate, the biomass content test and the metabolite testing, and finally the data processing. The result of this study indicate that the C3 treatment exhibited the highest growth rate, protein content (39.950 mg/mL ± 2,51b) and chlorophyll-a pigment (2.669 mg/mL ± 1.10b) compared to other treatments. The C1 treatment demonstrated the greatest increase in biomass content (1.561 mg/mL ± 0.0586b), lipids (0.850 mg/mL ± 0.02a) and carbohydrates (4.83 mg/L ± 0.22a) compared to other treatment. The results indicated that the addition of CAN (Calcium Ammonium Nitrate) fertilizer enhanced the growth rate and primary metabolites of Spirulina sp. semi-mass culture.

Kata Kunci : Calcium Ammonium Nitrate, Metabolisme, Protein, Semi-mass, Spirulina sp.