POTENSI ANTI BAKTERI MULTI-STRAIN PROBIOTIK Lactiplantibacillus plantarum subsp. plantarum (STRAIN Dad-13 dan FNCC-0250) dan Lacticaseibacillus paracasei GMRMP-001 TERHADAP Escherichia coli FNCC-0091 SECARA IN VITRO DAN IN VIVO

Devi Ariska, Yunika Mayangsari, S.Si., M.Biotech., Ph.D ; Prof. Dr. Ir. Endang Sutriswati Rahayu., M.S.

2024 | Tesis | S2 Ilmu dan Teknologi Pangan

Lactiplantibacillus plantarum subsp. plantarum Dad-13, Lactiplantibacillus subsp. plantarum plantarum FNCC-0250 dan Lacticaseibacillus paracasei GMRMP-001 merupakan strain probiotik yang diisolasi dari makanan fermentasi tradisional Indonesia. Penelitian ini bertujuan untuk mengevaluasi pemberian probiotik multi-strain dosis 10^-9 CFU/ml/hari selama 14 hari terhadap bakteri penyebab diare Escherichia coli FNCC-0091 dengan dosis 10^-11 CFU/ml/hari. Tiga puluh ekor mencit dibagi secara acak menjadi lima kelompok, yaitu kelompok kontrol, kelompok susu skim, kelompok single-strain, kelompok multi-strain, dan kelompok E. coli. Aktivitas antibakteri dianalisis secara in vitro, konsumsi pakan dan berat badan, analisis mikroba pada feses dan sekum, analisis short chain fatty acid dan pengujian morfologi usus tikus. Hasil penelitian menunjukkan bahwa zona hambat single-strain dan multi-strain pada uji in vitro menghasilkan potensi hambat yang sangat kuat (>9 mm). Jumlah L. plantarum dan L. paracasei meningkat dan jumlah E. coli menurun secara signifikan pada feses dan sekum mencit yang menunjukkan tingkat kelangsungan hidup bakteri di saluran pencernaan dan kemampuannya melawan bakteri patogen. Konsentrasi asam asetat, asam propionat, dan asam butirat juga mengalami peningkatan yang menunjukkan bahwa saluran pencernaan berada pada pH rendah sehingga dapat menghambat pertumbuhan bakteri patogen pada tikus. Selain itu, konsumsi probiotik multi-strain tidak memberikan efek negatif pada morfologi usus. Kesimpulannya adalah probiotik multi-strain mampu menghambat bakteri patogen secara in vivo dan in vitro.

Lactiplantibacillus plantarum Dad-13, Lactiplantibacillus plantarum FNCC-0250 and Lacticaseibacillus paracasei GMRMP-001 are probiotics strain isolated from Indonesian traditional fermented foods. This study aims to evaluate the administration of multi-strain probiotics at dose of 10^-9 CFU/ml/day for 14 days against the diarrhea-causing bacteria Escherichia coli FNCC-0091 at a dose of 10^-11 CFU/ml/day. Thirty mices were randomly divided into five groups, including control group, skim milk group, single-strain group, multi-strain group, and E. coli group. Antibacterial activity was analyzed in vitro, feed intake, body weight was analyzed, microbial analysis was carried out on feces and caecum, short chain fatty acids were analyzed and intestinal morphology was measured. The result showed that the single-strain and multi-strain inhibition zone in in vitro test produces a very strong inhibitory potential (>9 mm). The number of L. plantarum and L. paracasei increased and the number of E. coli decreased significantly in the feces and cecum of mice indicating the survival rate of the bacteria in the digestive tract and their ability to fight pathogenic bacteria. The concentration of acetic acid, propionic acid, and butyric acid also increased, indicating that the digestive tract is at a low pH so that it can inhibit the growth of pathogenic bacteria in rats. In addition, consumption of multi-strain probiotics does not have a negative effect and intestinal morphology. The conclusion is that multi-strain probiotics are able to inhibit pathogenic bacteria in vivo and in vitro.

Kata Kunci : Escherichia coli, Multi-strain, Probiotik, Short Chain Fatty Acid / Escherichia coli, Multi-strain, Probiotics, Short Chain Fatty Acid