Produktivitas, Asam Lemak Rantai Pendek, Histomorfologi Jejunum, dan Ekspresi Gen Tight Junction Broiler yang Diberi Pakan Sorgum Fermentasi dengan Menggunakan Limosilactobacillus fermentum BN21
Patrick Erhard Latue, Dr. Ir. Muhsin Al Anas, S.Pt., IPP.; drh. Bambang Ariyadi, M.P., Ph.D.
2024 | Tesis | S2 Ilmu PeternakanPenelitian ini bertujuan untuk mengetahui potensi penggunaan sorgum fermentasi terhadap produktivitas, produksi asam lemak rantai pendek (SCFA) di sekum, morfologi usus halus (jejunum), dan ekspresi gen tight junction (TJ) pada ayam broiler. Sebanyak 240 day-old chicks (DOC) broiler strain Indian River ditempatkan secara acak dalam lima perlakuan: kontrol (KTRL) dengan pakan basal, 10% sorgum tanpa fermentasi (STF10), 20% sorgum tanpa fermentasi (STF20), 10% sorgum fermentasi (SF10), dan 20% sorgum fermentasi (SF20). Setiap perlakuan terdiri dari 6 ulangan dengan 8 ekor ayam. Pemeliharaan dilakukan selama 35 hari dengan pakan dan minum ad libitum. Data performa yang diambil meliputi konsumsi pakan, pertambahan bobot badan, konversi pakan, mortalitas, dan indeks performa. Satu ekor ayam diambil dari setiap pen pada akhir pemeliharaan. Sampel digesta sekum digunakan untuk analisis produksi SCFA, sementara sampel jejunum digunakan untuk analisis histomorfologi dan ekspresi gen tight junction. Data dianalisis dengan analisis variansi mengikuti Rancangan Acak Lengkap dengan pola searah dan uji lanjut menggunakan uji orthogonal contrast bila terdapat perbedaan nyata. Hasil penelitian menunjukkan bahwa penambahan 20% sorgum fermentasi dapat meningkatkan konsumsi pakan, bobot badan, dan pertambahan bobot badan (P<0>P<0>P>0,05). Pemberian sorgum fermentasi dapat meningkatkan ekspresi gen occludin (OCLN) (P<0>claudin-1 (CLND-1), junctional adhesion molecule-2 (JAM-2), maupun zonula occludens-1 (ZO-1) (P>0,05). Kesimpulan penelitian ini adalah substitusi 20% sorgum dalam pakan dapat meningkatkan performa pertumbuhan, morfologi usus, dan ekspresi gen tight junction pada ayam broiler.
This study aimed to evaluate the potential of fermented sorghum on productivity, short-chain fatty acid (SCFA) production in the cecum, small intestine (jejunum) morphology, and tight junction (TJ) gene expression in broiler chickens. A total of 240 day-old chicks (DOC) of the Indian River broiler strain were randomly assigned to five treatments: control (KTRL) with a basal diet, 10% unfermented sorghum (STF10), 20% unfermented sorghum (STF20), 10?rmented sorghum (SF10), and 20?rmented sorghum (SF20). Each treatment consisted of six replicates with eight chickens per replicate. The rearing period lasted 35 days with ad libitum access to feed and water. Performance data collected included feed intake, body weight gain, feed conversion ratio, mortality, and performance index. One chicken from each pen was sampled at the end of the rearing period. Cecal digesta samples were analyzed for SCFA production, while jejunum samples were analyzed for histomorphology and tight junction gene expression. Data were analyzed using analysis of variance (ANOVA) following a completely randomized design with a one-way pattern. If significant differences were found, orthogonal contrast tests were performed for further analysis. The results showed that adding 20?rmented sorghum significantly increased feed intake, body weight, and body weight gain (P<0>P<0>P>0.05). The inclusion of fermented sorghum significantly upregulated the expression of the occludin (OCLN) gene (P<0>P>0.05). In conclusion, substituting 20% of the diet with fermented sorghum can enhance growth performance, intestinal morphology, and tight junction gene expression in broiler chickens.
Kata Kunci : sorgum, fermentasi, morfologi usus, scfa, ekspresi gen.