DETEKSI METHICILLIN RESISTANT Staphylococcus aureus (MRSA) DAN METHICILLIN RESISTANT Staphylococcus pseudintermedius (MRSP) ISOLAT ASAL PASIEN KUCING DAN ANJING DI "drh. NUGROHO ANIMAL CENTER SEMARANG"

SRI HANDAYANINGTYAS, Prof. Dr. drh. Siti Isrina Oktavia Salasia

2024 | Skripsi | KEDOKTERAN HEWAN

Menyebarnya strain methicillin-resistant Staphylococcus aureus (MRSA) dan methicillin-resistant Staphylococcus pseudintermedius (MRSP) merupakan bentuk resistensi bakteri terhadap antibiotik, terutama antibiotik beta-laktam yang menimbulkan ancaman terhadap kesehatan manusia dan hewan di seluruh dunia. Staphylococcus aureus dan Staphylococcus pseudintermedius merupakan bakteri yang mengkolonisasi kulit dan mukosa yang dapat menyebabkan infeksi oportunistik pada manusia dan hewan. Tujuan dari penelitian ini adalah mendeteksi MRSA dan MRSP secara fenotipik dengan metode oxacillin resistance screening agar base (ORSAB) dan secara genotipik terhadap keberadaan gen blaZ dan gen mecA dengan teknik Polymerase Chain Reaction (PCR).
Penelitian ini menggunakan 22 isolat, yang terdiri atas 18 isolat S. aureus dan 4 isolat S. pseudintermedius asal pasien kucing dan anjing dari “drh. Nugroho Animal Center Semarang”. Deteksi MRSA dan MRSP dilakukan dengan uji fenotipik menggunakan media ORSAB serta konfirmasi genotipik melalui amplifikasi gen blaZ dan gen mecA sebagai gen pengkode resistensi antibiotik dengan teknik PCR. Hasil amplifikasi gen dianalisis dengan teknik elektroforesis.
Hasil penelitian menunjukkan bahwa 15 isolat (83,33%) S. aureus teridentifikasi sebagai strain MRSA dan 3 isolat (16,67%) S. aureus teridentifikasi sebagai strain methicillin-susceptible Staphylococcus aureus (MSSA). Sebanyak 3 isolat (75%) S. pseudintermedius teridentifikasi sebagai strain MRSP dan 1 isolat (25%) S. pseudintermedius teridentifikasi sebagai strain methicillin-susceptible Staphylococcus pseudintermedius (MSSP).

The spread of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains is a form of bacterial resistance to antibiotics, especially beta-lactam antibiotics, which pose a threat to human and animal health worldwide. Staphylococcus aureus and Staphylococcus pseudintermedius are bacteria that colonize the skin and mucosa that cause opportunistic infections in humans and animals. This study aimed to detect MRSA and MRSP phenotypically using the oxacillin resistance screening agar base (ORSAB) method and genotypically for the presence of the blaZ gene and mecA gene using the Polymerase Chain Reaction (PCR) technique.
This study used 22 isolates, consisting of 18 S. aureus isolates and 4 S. pseudintermedius isolates from cat and dog patients from “drh. Nugroho Animal Center Semarang”. Detection of MRSA and MRSP was carried out by phenotypic testing using ORSAB media and genotypic confirmation through amplification of the blaZ gene and mecA gene as genes encoding antibiotic resistance using PCR techniques. Gene amplification results were analyzed using electrophoresis techniques.
The results showed that 15 isolates (83.33%) of S. aureus were identified as MRSA strains and 3 isolates (16.67%) of S. aureus were identified as methicillin-susceptible Staphylococcus aureus (MSSA) strains. A total of 3 isolates (75%) of S. pseudintermedius were identified as MRSP strains and 1 isolate (25%) of S. pseudintermedius was identified as methicillin-susceptible Staphylococcus pseudintermedius (MSSP) strains.

Kata Kunci : MRSA, MRSP, ORSAB, gen blaZ, gen mecA