Uji Sensitivitas Staphylococcus aureus dan Staphylococcus pseudintermedius Isolat Asal Anjing dan Kucing dari “drh. Nugroho Animal Center Semarang” terhadap berbagai Antibiotik

STIFANI KHAMIDAH, Prof. Dr. drh. Siti Isrina Oktavia Salasia

2024 | Skripsi | KEDOKTERAN HEWAN

Staphylococcus aureus (S. aureus) dan Staphylococcus pseudintermedius (S.pseudintermedius) merupakan dua spesies bakteri dari Genus Staphylococcus yang menjadi perhatian di dunia kedokteran hewan, khususnya terkait infeksi pada hewan peliharaan. Antibiotik merupakan pengobatan yang umum diberikan pada infeksi bakteri tersebut, tetapi terdapat strain S. aureus dan S. pseudintermedius yang resisten terhadap berbagai antibiotik sehingga mempersulit pemilihan pengobatan yang akan digunakan. Tujuan penelitian ini adalah untuk melihat pola resistensi Staphylococcus aureus dan Staphylococcus pseudintermedius yang diisolasi dari anjing dan kucing terhadap beberapa antibiotik.

Sampel yang digunakan dalam penelitian adalah Staphylococcus aureus dan S. pseudintermedius berjumlah 22 isolat yang diisolasi dari swab pasien anjing dan kucing dari “drh. Nugroho Animal Center Semarang”. Sampel diuji sensitivitasnya terhadap 8 (delapan) antibiotik menggunakan metode disk diffusion Kirby-Bauer. Media Muller-Hinton Agar (MHA) diinokulasikan dengan S. aureus dan S. pseudintermedius dan didiamkan selama 10-15 menit. Disk antibiotik diletakkan dalam media dan diinkubasi selama 18-24 jam pada suhu 37 ?C. Zona hambat yang terbentuk diukur dan diinterpretasikan berdasarkan standar Kirby-Bauer. Data yang diperoleh dianalisis secara deskriptif kualitatif.

Hasil uji sensitivitas S. aureus isolat asal anjing dan kucing telah resisten terhadap antibiotik tetrasiklin (75,50%/83,33%), penisilin dan ampisilin (75,00%/50,00%), oksasilin (58,33%/66,67%), klindamisin (16,67%/16,67%), eritromisin serta gentamisin (16,67%/0%). S. pseudintermedius isolat asal anjing telah resisten terhadap antibiotik ampisilin, penisilin, tetrasiklin (100%) serta oksasilin (66,67%). Sedangkan, S. pseudintermedius isolat asal kucing masih sensitif terhadap semua antibiotik yang digunakan.

Staphylococcus aureus (S. aureus) and Staphylococcus pseudintermedius (S. pseudintermedius) are two bacterial species of the Staphylococcus genus that are of concern in veterinary medicine, particularly regarding infections in pets. Antibiotics are commonly used in the treatment of these bacterial infections, but there are strains of S. aureus and S. pseudintermedius that are resistant to various antibiotics, making the selection of appropriate treatment challenging. The aim of this study was to observe the resistance patterns of Staphylococcus aureus and Staphylococcus pseudintermedius isolated from dogs and cats against several antibiotics.

The samples used in the study consisted of 22 isolates of S. aureus and S. pseudintermedius isolated from swabs taken from canine and feline patients at "drh. Nugroho Animal Center Semarang". The samples were tested for sensitivity to 8 (eight) antibiotics using the Kirby-Bauer disk diffusion method. Muller-Hinton Agar (MHA) medium was inoculated with S. aureus and S. pseudintermedius and left for 10-15 minutes. Antibiotic disks were placed on the medium and incubated for 18-24 hours at 37°C. The resulting inhibition zones were measured and interpreted based on Kirby-Bauer standards. The data obtained were analyzed descriptively and qualitatively.

The sensitivity test results of S. aureus isolates from dogs and cats showed resistance to tetracycline (75.50%/83.33%), penicillin and ampicillin (75.00%/50.00%), oxacillin (58.33%/66.67%), clindamycin (16.67%/16.67%), erythromycin, and gentamicin (16.67%/0%). S. pseudintermedius isolates from dogs were resistant to ampicillin, penicillin, tetracycline (100%), and oxacillin (66.67%). Meanwhile, S. pseudintermedius isolates from cats remained sensitive to all antibiotics used.

Kata Kunci : Staphylococcus aureus, Staphylococcus pseudintermedius, antibiotik, resistensi/ Staphylococcus aureus, Staphylococcus pseudintermedius, antibiotics, resistance