Deteksi Ion Fluorida Berbasis Turn-On Fluorescence Menggunakan Metal-Organic Frameworks (MOFs) UiO-66-NH2 Terfungsionalisasi Histidin
Muh Rizal B, Prof. Drs. Mudasir, M.Eng., Ph.D
2024 | Tesis | S2 Ilmu Kimia
Fungsionalisasi UiO-66-NH2 dengan histidin membentuk UiO-66-NH2@Histidin telah berhasil dilakukan dengan metode solvotermal. Keberhasilan hasil sintesis telah terkonfirmasi dengan munculnya puncak khas pada 2 theta sekitar 7,46o (111); 8,52o (200) dan 25,81o (600) pada difraktogram X-Ray Diffraction (XRD), serta dibuktikan dengan spektra Fourier Transform Infrared Spectroscopy (FTIR). UiO-66-NH2@Histidin juga telah dikarakterisasi dengan instrumen Field Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM), Surface Area Analyzer (SAA), Spektrofotometer UV-Vis, dan Spektrofluorometer.
Hasil penelitian menunjukkan bahwa kondisi optimum konsentrasi UiO-66-NH2@Histidin terhadap deteksi ion fluorida adalah 2 mg mL-1 dengan respon time 1 menit. Deteksi ion fluorida memberikan respon intensitas fluoresensi terbaik pada media air dan tidak ada respon pada pelarut non polar. Deteksi pada pH 3-10 memberikan respon intensitas fluoresensi yang relatif sama dan mengalami penurunan yang cukup signifikan pada pH 11-13. UiO-66-NH2@Histidin sangat selektif dan stabil terhadap ion fluorida yang dibuktikan intensitas fluoresensi tertinggi dan munculnya fenomena penderan berwarna biru pada larutan dibawah sinar UV lamda 365 nm apabila dibandingkan dengan anion-kation lainnya. Nilai LoD yang diperoleh pada penelitian ini adalah 0,011 ppm, dimana nilai tersebut lebih kecil dari konsentrasi maksimum ion fluorida dalam sampel air minum menurut World Health Organization (WHO) yaitu 1,5 ppm, sehingga material UiO-66-NH2@Histidin dapat diaplikasikan langsung pada sampel air minum.
Functionalization of UiO-66-NH2 with histidine to form UiO-66-NH2@Histidine has been successfully carried out using the solvothermal method. The success of the synthesis results has been confirmed by the appearance of a characteristic peak at 2 theta around 7.46o (111); 8.52o (200) and 25.81o (600) on the X-Ray Diffraction (XRD) diffractogram, and proven by Fourier Transform Infrared Spectroscopy (FTIR) spectra. UiO-66-NH2@Histidine has also been characterized using Field Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM), Surface Area Analyzer (SAA), UV-Vis Spectrophotometer, and Spectrofluorometer instruments.
The
research results showed that the optimum concentration conditions for UiO-66-NH2@Histidine
for fluoride ion detection were 2 mg mL-1 with a response time of 1 minute.
Fluoride ion detection provides the best fluorescence intensity response in
water media and no response in non-polar solvents. Detection at pH 3-10 gave a
relatively similar fluorescence intensity response and experienced a
significant decrease at pH 11-13. UiO-66-NH2@Histidine is very
selective and stable towards fluoride ions as evidenced by the highest
fluorescence intensity and the appearance of a blue luminescence phenomenon in
solution under lamda 365 nm UV light when compared to other ions. The LoD value
obtained in this study was 0.011 ppm, which is smaller than the maximum
concentration of fluoride ions in drinking water samples according to the World
Health Organization (WHO), namely 1.5 ppm so that the UiO-66-NH2@Histidine
material can be applied directly in drinking water samples.
Kata Kunci : fluorosensi, histidin, ion fluorida, UiO-66-NH2
