Pemberian Probiotik Bacillus spp., Lactococcus raffinolactis, Saccharomyces cerevisiae dan Imunostimulan Gama Algin F pada Pakan untuk Meningkatkan Kekebalan Non-Spesifik Seluler Lele (Clarias sp.)
MUHAMMAD ABRIANTO ALFARUQ, Prof. Dr. Ir. Alim Isnansetyo, M.Sc.
2024 | Skripsi | BUDIDAYA PERIKANAN
Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian probiotik Bacillus spp, Lactococcus raffinolactis, dan Saccharomyces cerevisiae, serta imunostimulan Gama Algin F terhadap kekebalan non-spesifik seluler ikan lele (Clarias sp.). Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan satu perlakuan kontrol dan tiga perlakuan kombinasi masing-masing tiga ulangan. Perlakuan yang diberikan yaitu A0B0 (kontrol), A1B0 (suplementasi probiotik tanpa imunostimulan), A0B1 (suplementasi imunostimulan tanpa probiotik), dan A1B1 (suplementasi probiotik dan imunostimulan). Parameter yang diamati adalah aktivitas fagositosis, indeks fagositosis, diferensiasi leukosit, total eritrosit, total leukosit, hematokrit, dan leukokrit. Hasil menunjukkan bahwa pemberian probiotik dan imunostimulan mampu meningkatkan aktivitas fagositosis, jumlah monosit, dan jumlah trombosit, tetapi tidak mampu meningkatkan indeks fagositosis, jumlah limfosit, neutrofil, eusinofil, basofil, hematokrit, dan leukokrit. Signifikansi pada efek utama terdapat pada aktivitas fagositosis dan jumlah monosit. Signifikansi pada efek interaksi terdapat pada aktivitas fagositosis, jumlah limfosit, dan jumlah trombosit, sedangkan signifikansi pada efek sederhana terdapat pada aktivitas fagositosis, jumlah monosit, jumlah limfosit, dan jumlah trombosit. Total eritrosit dan leukosit yang dianalisis secara deskriptif menunjukkan berada pada kisaran normal. Berdasarkan hasil yang didapatkan, dapat diketahui bahwa suplementasi pakan dengan probiotik Bacillus spp., Lactococcus raffinolactis, dan Saccharomyces cerevisiae, serta imunostimulan Gama Algin F mampu meningkatkan kekebalan non-spesifik seluler ikan lele (Clarias sp.) dan perlakuan imunostimulan tanpa probiotik (A0B1) memiliki hasil paling efektif untuk meningkatkan sistem kekebalan non-spesifik seluler.
The aims of this research were to determine the administration effect of probiotic Bacillus spp., Lactococcus raffinolactis, and Saccharomyces cerevisiae, and immunostimulant Gama Algin F on non-spesific cellular immunity of catfish (Clarias sp.). The research was conducted using a Factorial Completely Randomized Design (FCRD) with one control treatment and three combination treatments with three replications. The treatments were A0B0 (control), A1B0 (probiotic supplementation without immunostimulant), A0B1 (immunostimulant supplementation without probiotic), and A1B1 (probiotic and immunostimulant supplementation). The parameters observed were phagocytic activity, phagocytic index, leukocyte differentiation, total erythrocytes, total leukocytes, hematocrit and leukocrit. The results showed that administration of probiotics and immunostimulants was able to increase phagocytic activity, the number of monocytes, and the number of thrombocytes, but was not able to increase the phagocytic index, the number of lymphocytes, neutrophils, eucinophils, basophils, hematocrit, and leukocrit, as well as total erythrocytes and leukocytes. The significance of the main effect is in phagocytic activity and monocyte count. The significance of the interaction effect was found in phagocytic activity, lymphocytes count, and thrombocytes count, while the significance of the simple effect was found in phagocytic activity, monocytes count, lymphocytes count, and thrombocytes count. Total erythrocytes and leukocytes analyzed descriptively showed in the normal range. Based on the results obtained, it can be seen that feed supplementation with probiotics Bacillus spp., Lactococcus raffinolactis, and Saccharomyces cerevisiae, and immunostimulant Gama Algin F was able to increase the non-specific cellular immunity of catfish (Clarias sp.) and treatment of immunostimulant without probiotic (A0B1) has the most effective to improve the non-spesific cellular immune system.
Kata Kunci : Clarias sp., fagositosis, Gama Algin F, probiotik, respon imun non-spesifik
