Optimalisasi Konsentrasi Ekstrak Khamir Dalam Media Pertumbuhan Sel Lactiplantibacillus plantarum subsp. plantarum Dad-13 Pada Proses Produksi Bubuk Probiotik
JIHAN IFADAH, Prof. Dr. Ir. Endang Sutriswati Rahayu, M.S. ; Intan Rahmayanti, S.Si., M.Si.
2024 | Skripsi | TEKNOLOGI PANGAN & HASIL PERTANIAN
Modifikasi media pertumbuhan untuk bakteri asam laktat khususnya strain L.plantarum Dad-13 telah banyak dilakukan. Penggunaan ekstrak yeast sebagai dalam media fermentasi telah banyak digunakan serta terbukti memberikan pertumbuhan sel yang lebih cepat. Namun, dalam proses produksi perlu dilakukan penghematan bahan baku agar proses berjalan secara efisien. Tujuan dari penelitian ini adalah untuk mempelajari pengaruh optimalisasi konsentrasi ekstrak yeast terhadap pertumbuhan Lactiplantibacillus plantarum subsp. plantarum Dad-13 dalam media pertumbuhan pada proses produksi bubuk probiotik. Media pertumbuhan dengan penambahan konsentrasi ekstrak yeast 2,0 persen (sesuai rekomendasi) digunakan sebagai media pembanding. Media pertumbuhan terdiri dari pepton ikan gabus, ekstrak yeast, sukrosa, dan air. Untuk mengetahui pengaruh konsentrasi ekstrak yeast, kultur L.plantarum Dad-13 ditumbuhkan dalam media pertumbuhan dengan berbagai variasi konsentrasi ekstrak yeast (0,5 persen; 1,0 persen; 1,5 persen) pada suhu 30 derajat celsius selama 20 jam dan pertumbuhan sel diukur dalam CFU/ml. Hasil penelitian menunjukkan bahwa penambahan ekstrak yeast sebesar 1,0 persen (10 g/L) dalam media pertumbuhan menghasilkan jumlah sel Lactiplantibacillus plantarum subsp. plantarum Dad-13 sebesar 1,05 x 10? CFU/ml yang sudah mendekati media kontrol dan paling efisien dalam proses produksi sel.
Modification of growth media for lactic acid bacteria, especially L.plantarum Dad-13 strain, has been widely done. The use of yeast extract as a fermentation medium has been widely used and found to provide faster cell growth. However, in the production process, it is important to save raw materials so that the process runs efficiently. The purpose of this research is to study the effect of optimizing the concentration of yeast extract on the growth of Lactiplantibacillus plantarum subsp. plantarum Dad-13 in growth media in the production process of probiotic powder. Growth media with the addition of 2,0 percen yeast extract concentration (as recommended) was used as a comparison media. The growth medium consisted of snakehead fish waste peptone, yeast extract, sucrose, and water. To determine the effect of yeast extract concentration, L.plantarum Dad-13 culture were grown in growth media with various yeast extract concentration (0,5 percen; 1,0 percen; 1,5 percen) at 30 degree celsius for 20 hours and cell growth was measured in CFU/ml. The result showed that the addition of yeast extract at 1,0 percen (10 g/L) in the growth medium resulted in a cell count of Lactiplantibacillus plantarum subsp. plantarum Dad-13 of 1,05 x 10? CFU/ml which was close to the control growth medium and most efficient in the cell production process.
Kata Kunci : bakteri asam laktat, Lactiplantibacillus plantarum subsp. plantarum Dad-13, media pertumbuhan, ekstrak yeast, pertumbuhan sel / lactic acid bacteria, Lactiplantibacillus plantarum subsp. plantarum Dad-13, growth medium, yeast extract, cell growth