ISOLATION AND IDENTIFICATION OF SECONDARY METABOLITES PRODUCED BY Kaempferia galanga L.
Luthfiana Indah Hastuti, Dr. Widiastuti Setyaningsih, S.T.P., M.Sc ; Prof. Yoshihito Shiono
2024 | Tesis | S2 Ilmu dan Teknologi Pangan
Kaempferia galanga, a plant with numerous undiscovered compounds, holds great potential in the food industry. These compounds can enhance the nutritional value of food products, serve as natural antioxidants and preservatives, and extend the shelf life of food items. To uncover these novel compounds from raw materials, an initial step of isolation and identification is essential, enabling subsequent stages such as biological activity analysis for the utilization of these compounds in the food and pharmaceutical industries. This study aims to isolate and identify secondary metabolites from the extract of Kaempferia galanga L. from Indonesia using column chromatography. The identification of compounds is conducted through 1H NMR analysis. The results from the separating funnel using chloroform indicate a higher extract yield based on both crude weight and volume, suggesting that chloroform possesses a moderate level of polarity and its solubility characteristics are more suitable for the extraction of compounds. The plant extract underwent chloroform extraction, followed by separation using column chromatography with hexane-ethyl acetate and chloroform-ethyl acetate solvents. Compound 1 (ethyl p-methoxycinnamate) was isolated in the first stage, while compounds 2 (methyl p-coumarate) and compound 3 (2-methoxyphenol) were obtained in the second stage. Methyl p-coumarate and 2-methoxyphenol are known compounds, but they are newly discovered within the extract of Kaempferia galanga.
Kaempferia galanga, a plant with numerous undiscovered compounds, holds great potential in the food industry. These compounds can enhance the nutritional value of food products, serve as natural antioxidants and preservatives, and extend the shelf life of food items. To uncover these novel compounds from raw materials, an initial step of isolation and identification is essential, enabling subsequent stages such as biological activity analysis for the utilization of these compounds in the food and pharmaceutical industries. This study aims to isolate and identify secondary metabolites from the extract of Kaempferia galanga L. from Indonesia using column chromatography. The identification of compounds is conducted through 1H NMR analysis. The results from the separating funnel using chloroform indicate a higher extract yield based on both crude weight and volume, suggesting that chloroform possesses a moderate level of polarity and its solubility characteristics are more suitable for the extraction of compounds. The plant extract underwent chloroform extraction, followed by separation using column chromatography with hexane-ethyl acetate and chloroform-ethyl acetate solvents. Compound 1 (ethyl p-methoxycinnamate) was isolated in the first stage, while compounds 2 (methyl p-coumarate) and compound 3 (2-methoxyphenol) were obtained in the second stage. Methyl p-coumarate and 2-methoxyphenol are known compounds, but they are newly discovered within the extract of Kaempferia galanga.
Kata Kunci : NMR, Zingiberaceae, Extraction
