Efek Penambahan Limbah Cair Industri Rumah Potong Ayam terhadap Pertumbuhan dan Kemampuan Rothia Sp. SO3K dalam Mengurai Limbah Organik

Herlinda Arum Dewantari, Prof. Ir. Nanung Agus Fitriyanto, S.Pt., M.Sc., Ph.D., IPM

2024 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui perilaku pertumbuhan isolat bakteri Rothia sp. SO3K dalam limbah cair RPA dan kemampuanya dalam mengurai bahan organik melalui proses nitrifikasi - denitrifikasi secara aerobik dengan menjadi agen bioremediasi. Penelitian dimulai dengan menumbuhkan Rothia sp. SO3K pada medium padat dan cair dengan penambahan limbah cair RPA dengan konsentrasi 0%, 25%, 50%, 75%, dan 100%. Viabilitas bakteri juga dilihat dengan menginokulasikan bakteri yang telah diinkubasi dalam medium limbah cair selama 24 dan 48 jam pada medium nutrien padat. Kemampuan Rothia sp. SO3K untuk mereduksi amonia, nitrat dan bahan organik limbah cair RPA diuji sebelum dan sesudah proses aerasi selama empat hari. Data yang didapatkan yaitu pertumbuhan bakteri, viabilitas bakteri, nilai total solid (TS), nilai total volatile solid (TVS), nilai total suspended solid (TSS), nilai total dissolved solid (TDS), biological oxygen demand (BOD5), chemical oxygen demand (COD), kadar amonia dan kadar nitrat. Data hasil penelitian dianalisis secara statistik menggunakan variasi rancangan acak lengkap (RAL) pola searah yang dilanjutkan dengan uji beda Duncan’s Multiple Range Test (DMRT). Hasil yang didapatkan menunjukkan bahwa Rothia sp. SO3K dapat tumbuh di medium cair maupun padat pada setiap perlakuan. Presentase penurunan terbesar untuk nilai TS yaitu 43,98 ± 2,182% pada B2, nilai TVS sebesar 35,10 ± 5,157% pada B3, nilai TDS sebear 42,54 ± 2,580% pada B4, nilai TSS sebesar -46,41 ± 8,988%c pada B3, nilai BOD5 66,63 ± 17,519% pada B4, untuk nilai COD yaitu 87,46 ± 5,377% pada B4, untuk nilai kadar amonia yaitu 11.913 ± 4.495% pada B3, dan kadar nitrat yaitu 25,62 ± 1,66% pada B3. Berdasarkan ini dapat disimpulkan bahwa bakteri Rothia sp. SO3K dapat menurunkan kadar bahan organik, kadar amonia dan kadar nitrat pada limbah cair RPA.

This research aims to understand the growth behavior of the Rothia sp. SO3K bacteria isolate in chicken slaughterhouse wastewate and its ability to degrade organic matter through the nitrification – denitrification process aerobically, acting as bioremediation agent. The research began cultivating Rothia sp. SO3K on solid and liquid medium with the addition of chicken slaughterhouse wastewater concentrations of 0%, 25%, 50%, 75%, and 100%. Bacterial viability was also observed by inoculating bacteria that had been incubated in liquid waste medium for 24 and 48 hours on solid nutrient medium. The ability of Rothia sp. SO3K to reduce amonia, nitrate, and organic matter in chicken slaughterhouse wastewater was tested before and after the aeration process for four days. The data collected included bacterial growth, bacterial viability, total solid (TS) value, total volatile solid (TVS) value, total suspended solid (TSS) value, total dissolved solid (TDS) value, biological oxygen demand (BOD5), chemical oxygen demand (COD), amonia content, and nitrate content. The research data were statistically analyzed using a complete randomized design (CRD) with a one-way pattern followed by Duncan's Multiple Range Test (DMRT). The results indicated that Rothia sp. SO3K could grow in both liquid and solid mediums under each treatment. The largest reduction percentage for TS value was 43.98 ± 2.182% in B2, TVS value was 35.10 ± 5.157% in B3, TDS value was 42.54 ± 2.580% in B4, TSS value was -46,41 ± 8,988%c in B3, BOD5 value was 66,63 ± 17,519% in B4, COD value was 87,46 ± 5,377% in B4, amonia value was 11.913± 4.495% in B3, and nitrate value was 25,62 ± 1,66% in B3. It can be concluded Rothia sp. SO3K could reduce the levels of organic matter, amonia, and nitrate in chicken slaughterhouse wastewate.

Kata Kunci : limbah cair, rumah potong ayam, Rothia sp., bioremediasi