Pengaruh Penambahan Probiotik Lactobacillus casei pada Gel Aloe vera Berbagai Konsentrasi Terhadap Daya Hambat Pertumbuhan Prevotella intermedia

ADDIEN MUTIARA AGMA, drg. Kwartarini Murdiastuti, Sp.Perio(K), Ph.D; drg. Vincensia Maria Karina, MDsc., Sp.Perio(K)

2024 | Skripsi | PENDIDIKAN DOKTER GIGI

Periodontitis adalah peradangan kronis pada periodonsium yang disebabkan oleh akumulasi plak di rongga mulut yang terdiri dari bakteri periodontopatogen, salah satunya Prevotella intermedia. Perawatan periodontitis, yaitu scaling dan root planing, kurang efektif dalam menghambat pertumbuhan bakteri yang berinvasi ke dalam jaringan sehingga diperlukan terapi adjuvan, salah satunya dengan pemberian Aloe vera yang mengandung senyawa antibakteri dan mampu penetrasi ke jaringan, namun masih perlu penambahan Lactobacillus casei untuk mencegah rekolonisasi bakteri periodontopatogen. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan probiotik Lactobacillus casei pada gel Aloe vera berbagai konsentrasi terhadap daya hambat pertumbuhan Prevotella intermedia.

Uji daya hambat Prevotella intermedia dilakukan menggunakan metode difusi cakram dengan tujuh kelompok uji diantaranya tiga kelompok perlakuan (kelompok gel Aloe vera 5%, 10%, 15% diperkaya Lactobacillus casei) dan empat kelompok kontrol (kontrol probiotik (salin diperkaya Lactobacillus casei), kontrol negatif (pelarut salin), kontrol MIC (gel Aloe vera 20%), dan kontrol positif (CHX)). Pengukuran diameter zona hambat dilakukan menggunakan sliding caliper dan dianalisis menggunakan uji One Way ANOVA dan Post Hoc Games-Howell.

Hasil penelitian menunjukkan bahwa terdapat perbedaan zona hambat yang signifikan pada ketiga kelompok perlakuan (p>0,05). Pada kelompok gel Aloe vera 5%, 10%, 15% diperkaya Lactobacillus casei terdapat peningkatan zona hambat seiring dengan peningkatan konsentrasi gel Aloe vera, meskipun daya hambat dari ketiga kelompok perlakuan lebih kecil dibandingkan kontrol MIC dan kontrol positif. Kesimpulan dari penelitian ini adalah penambahan probiotik Lactobacillus casei pada gel Aloe vera berbagai konsentrasi berpengaruh meningkatkan daya hambat pertumbuhan Prevotella intermedia.

Periodontitis is a chronic inflammation of the periodontium caused by the accumulation of plaque in the oral cavity consisting of periodontopathogenic bacteria, such as Prevotella intermedia. Treatment of periodontitis, scaling and root planing, is less effective in inhibiting the growth of bacteria that invade the tissues thus adjuvant therapy, such as the administration of Aloe vera which contains antibacterial compounds and capable of penetration into the tissue is needed, but still requires the addition of Lactobacillus casei to prevent the recolonization of the periodontopathogenic bacteria. This study aims to determine the influence of adding Lactobacillus casei probiotics at various concentrations of Aloe vera gel on the inhibitory effect on the growth of Prevotella intermedia.

The inhibition test of Prevotella intermedia was conducted using the disc diffusion method with seven test groups, including three treatment groups (Aloe vera gel at 5%, 10%, 15% enriched with Lactobacillus casei) and four control groups (probiotic control group (saline enriched with Lactobacillus casei), negative control group (saline solvent), MIC control group (Aloe vera gel 20%), and positive control group (CHX)). The measurement of the diameter of the inhibition zone was carried out using a sliding caliper and analyzed using One Way ANOVA and Post Hoc Games-Howell.

The results showed that there were significant differences in the inhibition zones in three treatment groups (p>0.05). In the Aloe vera gel groups at 5%, 10%, 15% enriched with Lactobacillus casei, there was an increase in the inhibition zone with the increasing concentration of Aloe vera gel, although the inhibitory power of the three treatment groups was smaller compared to the MIC control and positive control. The conclusion of this study is that the addition of Lactobacillus casei probiotics at various concentrations of Aloe vera gel has an effect on increasing the inhibitory effect on the growth of Prevotella intermedia.

Kata Kunci : gel Aloe vera, Lactobacillus casei, Prevotella intermedia, antibakteri, daya hambat