Pengaruh Daya Antibakteri Larutan Irigasi Eco-enzyme Limbah Jeruk Peras (Citrus sinensis L.) 10 Persen terhadap Pertumbuhan Treponema denticola

NADYA ALYA BANAFSAJ, drg. Hendrawati, M.Kes.; drg. Sri Pramestri Lastianny, MS., Sp.Perio(K)

2023 | Skripsi | PENDIDIKAN DOKTER GIGI

Penyakit periodontal adalah penyakit inflamasi kronis yang menyebar ke jaringan penyangga gigi dan dapat disebabkan oleh Treponema denticola (Td). Keberhasilan perawatan penyakit periodontal dapat ditingkatkan dengan melakukan terapi adjuvan, salah satunya dengan irigasi subgingiva. Eco-enzyme limbah jeruk peras (Citrus sinensis L.) memiliki sifat antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh daya antibakteri larutan irigasi eco-enzyme limbah jeruk peras (Citrus sinensis L.) 10% terhadap pertumbuhan Td. 

Metode yang digunakan untuk menguji pengaruh daya antibakteri larutan irigasi eco-enzyme limbah jeruk peras (Citrus sinensis L.) 10% terhadap pertumbuhan Td yaitu dengan metode difusi cakram Kirby-Bauer (disc diffusion) pada media pertumbuhan MHA. Larutan uji yang digunakan yaitu larutan irigasi eco-enzyme limbah jeruk peras (Citrus sinensis L.) 10%, klorheksidin 0,2% sebagai kontrol positif, dan akuades sebagai kontrol negatif. Masing-masing larutan uji terdiri dari sembilan sampel. Pengamatan dilakukan dengan mengukur diameter zona hambat, yaitu daerah bening di sekitar kertas cakram dengan sliding caliper. Data dianalisis dengan uji One-Way ANOVA dan Post Hoc LSD. 

Hasil penelitian menunjukkan terdapat zona hambat pada kelompok kontrol positif dan kelompok eco-enzyme 10%. Sementara pada kelompok kontrol negatif tidak terdapat zona hambat. Hasil analisis menunjukkan adanya pengaruh signifikan (p<0>uji terhadap diameter zona hambat pertumbuhan Td dan adanya perbedaan bermakna antarkelompok (p<0>. Kesimpulan penelitian ini adalah daya antibakteri larutan irigasi eco-enzyme limbah jeruk peras (Citrus sinensis L.) 10?rpengaruh menghambat pertumbuhan Treponema denticola. 

Periodontal disease is a chronic inflammatory disease that spreads to the supporting tissues of the teeth and can be caused by the Treponema denticola (Td). The success of periodontal disease treatment can be increased by carrying out adjuvant therapy, one of which is subgingival irrigation. Eco-enzyme from squeezed orange (Citrus sinensis L.) waste has antibacterial properties. The research aimed to determine the antibacterial effect of 10?o-enzyme irrigation solution from squeezed orange (Citrus sinensis L.) waste on the growth of Td. 

The method used to test the antibacterial effect of 10?o-enzyme irrigation solution from squeezed orange (Citrus sinensis L.) waste on the growth of Td was by the Kirby-Bauer disc diffusion method on MHA growth media. The test solutions used were 10?o-enzyme irrigation solution from squeezed orange (Citrus sinensis L.) waste, 0.2% chlorhexidine as a positive control, and aquadest as a negative control. Each test solution consisted of nine samples. Observations were made by measuring the diameter of the inhibition zone, namely the clear area around the paper disc with a sliding caliper. The data was analyzed using One-Way ANOVA test and Post Hoc LSD test. 

The result of study showed that there was an inhibition zone around positive control group and 10?o-enzyme group. Meanwhile, in the negative control group there was no inhibition zone. Analysis result showed there was a significant effect (p<0>Td and there was a significant difference between groups (p<0>The study concludes that the antibacterial effect of 10?o-enzyme irrigation solution from squeezed orange (Citrus sinensis L.) waste has an inhibitory effect on the growth of Treponema denticola. 

Kata Kunci : Treponema denticola, eco-enzyme, antibakteri, Citrus sinensis L., antibacterial