Respon Varian Genotip APOA1 rs670 dan APOA5 rs662799 Terhadap Intervensi Kukis FiberCreme Dan Efeknya Pada Indeks Aterogenik Plasma Subjek Hiperlipidemia

Wynda Lestari Lamaliwa, Prof. Dr.Dra. Sunarti, M.Kes; dr. Arta Farmawati, Ph.D

2023 | Tesis | S2 Ilmu Kedokteran Dasar dan Biomedis

Latar belakang: Hiperlipidemia merupakan salah satu faktor penyebab risiko penyakit kardiovaskular. Salah satu prediktor penyakit kardiovaskular yaitu indeks aterogenik plasma (IAP). Asupan nutrisi serat dari FiberCreme diyakini dapat memperbaiki profil lipid dan menurunkan nilai IAP. Namun, terapi nutrisi dapat direspon oleh varian genotip APOA1 rs670 dan APOA5 rs662799 sehingga menghasilkan efek yang berbeda. Tujuan penelitian: menganalisis respon varian genotip APOA1 dan APOA5 dengan konsumsi FiberCreme terhadap perubahan IAP. Metode: sebanyak 47 isolat DNA subjek hiperlipidemia yang telah diintervensi kukis FiberCreme selama 4 minggu lalu dianalisis genotip menggunakan metode PCR-RFLP. Hasil: konsumsi FiberCreme cenderung dapat menurunkan rerata nilai IAP tetapi tidak signifikan (p>0,05). Alel A setelah intervensi kukis FiberCreme protektif terhadap risiko penyakit kardiovaskular tetapi tidak signifikan secara statistik. Alel C setelah intervensi kukis FiberCreme protektif terhadap risiko penyakit kardiovaskular tetapi tidak signifikan secara statistik. Kesimpulan: kukis FiberCreme dapat menurunkan IAP pada subjek hiperlipidemia. Alel A setelah intervensi kukis FiberCreme menurunkan risiko penyakit kardiovaskular. Alel C setelah intervensi kukis FiberCreme menurunkan risiko penyakit kardiovaskular.

Background: Hyperlipidemia is one of the factors contributing to the risk of cardiovascular disease. One of the predictors of cardiovascular disease is the atherogenic index plasma (AIP). Dietary fiber intake from FiberCreme is believed to improve lipid profiles and reduce AIP values. However, nutritional therapy can be influenced by APOA1 rs670 and APOA5 rs662799 genotype variants, resulting in different effects. Research Objective: To analyze the response of APOA1 and APOA5 genotype variants to FiberCreme consumption on changes in AIP. Method: A total of 47 DNA isolates from hyperlipidemia subjects who underwent FiberCreme cookie intervention for 4 weeks, then were genotyped using the PCR-RFLP method. Results: FiberCreme consumption led to a decrease in the mean AIP value, although it was not statistically significant (p>0.05). The A allele after FiberCreme cookie intervention showed a protective effect against the risk of cardiovascular disease but was not statistically significant. The C allele after FiberCreme cookie intervention also showed a protective effect against the risk of cardiovascular disease but was not statistically significant. Conclusion: FiberCreme cookies can reduce AIP in hyperlipidemia subjects. The A allele after FiberCreme cookie intervention decreases the risk of cardiovascular disease. The C allele after FiberCreme cookie intervention also decreases the risk of cardiovascular disease.

Kata Kunci : Hiperlipiedemia, FiberCreme, APOA1 rs670, APOA5 rs662799, IAP