Polimorfisme Gen PRL terhadap Produktivitas Telur Ayam (Gallus gallus domesticus Linnaeus, 1758) F2 Mahkota Arab
Angga Bintang Narzassi, Prof. Dr. Budi Setiadi Daryono, M.Agr. Sc.
2023 | Skripsi | BIOLOGI
The demand for local chicken eggs in Indonesia continues to increase. However, the development of free-range chickens for food needs is still not optimal, due to low egg productivity. Hibrida Mahkota and Arabic chickens have the potential to be used as broodstock to produce superior local laying hens, because they have high productivity with egg characteristics like local chickens. The purpose of this study was to determine the relationship of indel polymorphism in the PRL promoter and to see its effect on egg productivity. The chickens used in this study were F2 Mahkota Arab hens as a result of a cross between hens of F1 Mahkota Arab and roosters of F1 Mahkota Arab as many 8 chickens. The stages of the research were rearing chickens, collecting egg data for 8 weeks, and testing egg quality. In addition, DNA isolation and PRL gene amplification were carried out with the primer in/del 358 forward '5-TTT AAT ATT GGT GGG TGA AGA GACA-3'; and reverse 5?-ATG CCA CTG ATC CTC GAA AAC TC-3?, and visualization of DNA bands. The results showed that the average number of eggs for F2 Mahkota Arab chickens for 8 weeks was 40.4 eggs, higher than the Pelung range chicken 37 eggs and almost the same as the Arab chicken 40 eggs. Inbreeding coefficient is 0,25 (25%). The average egg weight of F2 Mahkota Arab chickens was 37.27 g, the average yolk index was 0.37 ± 0.006, the average egg albumin index was 0.121 ± 0.005, the average yolk color was 7.40 ± 0.267, and the average haugh unit was 91.99 ± 1.13. Analysis with the Pearson correlation test showed that there was no correlation between indel polymorphism in the PRL promoter on the egg productivity of F2 Mahkota Arab chickens, because the significance value was 0.995 and the correlation value was 0.061 (P>0.05).
Kata Kunci : ayam Arab, ayam Mahkota, gen PRL, polimorfisme, produktivitas telur