Pengaruh Konsumsi Kukis Isomaltooligosakarida dan Genotipe PRKAA2 rs1124900 serta ABCA1 rs2066714 terhadap Castelli’s Risk Index Subjek Hiperlipidemia

Gharini Sumbaga Narhadina, Prof. Dr. Dra. Sunarti, M.Kes; dr. Arta Farmawati, Ph.D

2023 | Tesis | S2 Ilmu Kedokteran Dasar dan Biomedis

Latar Belakang: Hiperlipidemia dapat menjadi faktor resiko terjadinya penyakit jantung iskemik. Risiko jantung iskemik dapat diprediksi dengan castelli’s risk index 1 (CRI1). Variasi genotipe PRKAA2 rs1124900 dan ABCA1 rs2066714 berpengaruh terhadap perubahan lipid plasma. Asupan nutrisi isomaltooligosakarida (IMO) memiliki efek hipolipidemik. Tujuan Penelitian: penelitian ini menganalisis perubahan CRI1 pada subjek dengan variasi genetik PRKAA2 dan ABCA1 yang mengkonsumsi kukis IMO. Metode: 30 isolat DNA dari subjek hiperlipidemia yang telah diberi perlakuan kukis IMO selama 4 minggu. Analisis genotipe menggunakan PCR-RFLP dan enzim restriksi. Hasil Penelitian: Kukis IMO menyebabkan penurunan rerata CRI1 subjek hiperlipidemia (8,06%) (p<0>0,05). Kesimpulan: Kukis IMO menyebabkan penurunan CRI1 pada subjek hiperlipidemia. Konsumsi kukis IMO pada genotipe TG dan GG PRKAA2 rs1124900 dapat menurunkan resiko jantung iskemik.

Background: Hyperlipidemia can contribute to the development of ischemic heart disease, and Castelli's Risk Index 1 (CRI1) can help predict the risk. Genetic variations, such as PRKAA2 rs1124900 and ABCA1 rs2066714, can influence lipid levels in the blood. Moreover, the consumption of isomaltooligosaccharide (IMO) can have a beneficial effect on lowering lipid levels. Objectives: aims to analyze the changes in CRI1) in subjects with genetic variations who consume isomaltooligosaccharide (IMO) cookies. Method: In this study, 30 DNA isolates were collected from subjects with hyperlipidemia who were given a treatment of isomaltooligosaccharide (IMO) cookies for 4 weeks. Using PCR-RFLP and restriction enzyme digestion. Result: consumption of IMO cookies resulted in a significant decrease in CRI1 in subjects with hyperlipidemia (8.06%) (p<0>0.05). Conclusion: IMO cookies lead to a decrease in Castelli's Risk Index 1 (CRI1) in subjects with hyperlipidemia. The consumption of IMO cookies in TG and GG genotypes of PRKAA2 rs1124900 can reduce CRI1 ischemic heart disease risk. 

Kata Kunci : Hiperlipidemia, isomaltooligosakarida, PRKAA, ABCA1, CRI1/ Hyperlipidemia, isomaltooligosaccharide, PRKAA, ABCA1, CRI1