EVALUASI SISTEM PERTAHANAN NON-SPESIFIK NILA MERAH (Oreochromis sp.) HIBRIDA HASIL SELEKSI INDIVIDU

RAHMAT ADITAM, Dr. Ir. Murwantoko, M.Si.; Prof. Dr. Ir. Sutadi, M.Sc.

2012 | Skripsi | S1 PERIKANAN

Penelitian ini bertujuan untuk mengevaluasi sistem pertahanan non-spesifik nila merah top dan kontrol, hasil seleksi individu dari populasi sintetik hasil persilangan ikan nila strain Nifi, Filipina, Singapura dan Citralada. Pengamatan sistem pertahanan nonspesifik dilakukan dengan uji LD50, uji fagositosis, aktivitas NBT, leukokrit, dan penghitungan jumlah leukosit. Ikan nila hasil pendederan ke-2 diadaptasikan dengan lingkungan laboratorium. Setelah aklimatisasi, untuk diuji LD50, ikan diinfeksi bakteri Streptococcus sp. dan Aeromonas hydrophila dan dilakukan pengamatan kematian ikan selama 14 hari. Pengamatan aktifitas fagositosis, NBT, leukokrit dan penghitungan jumlah leukosit dilakukan dengan mengambil sampel darah melalui vena caudalis. Hasil penelitian menunjukan bahwa nilai rerata LD50 ikan nila top adalah 9,61 x 105 CFU/ml (Streptococcus sp.) dan 2,74 x 105 CFU/ml (Aeromonas hydrophila), sedangkan nilai rerata LD50 nila kontrol adalah 6,87 x 105 CFU/ml (Streptococcus sp.) dan 9,35 x 105 CFU/ml (Aeromonas hydrophila). Nilai rerata aktifitas fagositosis ikan nila top adalah 18,25% dan ikan nila kontrol adalah 15%, nilai rerata NBT ikan nila top adalah 0,64 dan ikan nila kontrol adalah 0,67 , nilai rerata leukokrit ikan nila top adalah 2,55 dan ikan nila kontrol adalah 2,12, penghitungan jumlah leukosit ikan nila top adalah 21696,25 sel/mm3 dan ikan nila kontrol adalah 20541,5 sel/mm3. Berdasarkan analisis varian tidak terdapat perbedaan yang nyata sistem pertahanan non-spesifik ikan nila top dengan ikan nila kontrol.

The objectives of this research was to evaluate non-specific immune response of red tilapia top and control, of individual selection from synthetic population of Nifi, Philipines, Singapore and Citralada hybrids. Observations of non-specific immune responses were investigated by LD50 test, phagocytic activity, nitroblue tetrazolium test, leucocrit, and leucocyte counts. Red tilapia produced from second rearing stage were adapted to the research environment. After that, for LD50 test, fish were infected with Streptococcus sp. and Aeromonas hydrophila and mortalities were recorded for 14 days. While observations of phagocytic activity, nitroblue tetrazolium test, leucocrit, and leucocyte counts were performed by taking samples of blood from caudal vein. The results of this study show that the average LD50 of red tilapia top were 9,61 x 105 CFU/ml (Streptococcus sp.) and 2,74 x 105 CFU/ml (A. hydrophila), whereas the average LD50 of red tilapia control were 6,87 x 105 CFU/ml (Streptococcus sp.) and 9,35 x 105 CFU/ml (A. hydrophila). The average phagocytic activity of red tilapia top were 18,25% and red tilapia control were 15%. The average nitroblue tetrazolium test of red tilapia top were 0.64 and red tilapia control were 0,67. The average leucocrit of red tilapia top were 2,55 and red tilapia control were 2,12. The average leucocyte counts of red tilapia top were 21696,25 sel/mm3 and red tilapia control were 20541,5 sel/mm3. There are no significant different of non-specific immune response between red tilapia top and control.

Kata Kunci : sistem pertahanan non-spesifik, ikan nila merah, hibrida