Korelasi Kadar Testosteron terhadap Testis Ayam Bangkok (Gallus gallus domesticus) yang diberi Intervensi Tepung Tulang Bandeng (Chanos chanos) Metode Freeze Drying

Krisnayan Adha, drh. Yuda Heru Fibrianto, M.P., Ph.D

2023 | Skripsi | KEDOKTERAN HEWAN

Limbah tulang ikan bandeng dapat dimanfaatkan menjadi suplemen pakan yang menunjang kebutuhan untuk perkembangan ayam bangkok. Penelitian ini bertujuan untuk mengetahui korelasi kadar testosteron serum ayam bangkok yang diberi tepung tulang bandeng terhadap panjang, lebar, dan berat testis ayam bangkok. Materi dalam penelitian ini menggunakan 9 ekor ayam bangkok yang dibagi menjadi kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan (P) terdiri dari 5 ekor ayam yang diberi perlakuan berupa pemberian suplemen tepung tulang ikan bandeng sebanyak 3,3 gram selama 5 minggu. Kelompok kontrol (K) tidak diberikan suplemen tepung tulang ikan bandeng. Serum darah ayam diambil setiap minggu dan diakhir masa penelitian ayam dinekropsi untuk diukur panjang, lebar, dan berat testis serta diuji kadar testosteron serumnya. Sampel serum darah diuji dengan metode competitive ELISA. Hasil penelitian menunjukkan bahwa ayam yang diberi suplemen tepung tulang ikan bandeng memiliki ukuran testis yang lebih tinggi dibanding ayam kontrol yang tidak yang diberi suplemen tepung tulang ikan bandeng. Hasil analisa uji korelasi Pearson dan Spearman menunjukkan adanya korelasi lemah kadar testosteron terhadap pertumbuhan panjang testis (0,20 < p>

Milkfish bone waste can be utilized as a feed supplement that supports the needs for the development of thai game fowl. This study aims to determine the correlation of serum testosterone levels of thai game fowl fed with milkfish bone meal on the length, width, and weight of the testes of thai game fowl. The material in this study used 9 thai game fowl which were divided into treatment groups and control groups. The treatment group (P) consisted of 5 fowls that were treated by supplementing milkfish bone powder as much as 3.3 grams for 5 weeks. The control group (K) was not supplemented with milkfish bone powder. Fowls blood serum was taken every week and at the end of the study period the fowl was necropsied to measure the length, width, and weight of the testicles and tested for serum testosterone levels. Blood serum samples were tested by competitive ELISA method. The results showed that fowls supplemented with milkfish bone powder had higher testicular measurement than control fowls that were not supplemented with milkfish bone powder. The results of Pearson and Spearman correlation test analysis showed a weak correlation of testosterone levels on testicular length growth (0.20 < p>

Kata Kunci : Ayam bangkok, ELISA, ikan bandeng, testis, testosteron