Penngaruh Induksi Mutagen Kimia Colchicine Terhadap Karakteristik Morfologi dan Produksi Biomasa Rumput Ruzi (Brachiaria ruziziensis cv. Kennedy)

Efryda Zelina Wardhani, Prof. Ir. Nafiatul Umami, S.Pt., M.P., Ph.D., IPM., ASEAN Eng.

2023 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh induksi senyawa colchicine  terhadap morfologi dan produksi biomasa rumput Ruzi (Brachiaria ruziziensis cv. Kennedy). Penelitian ini dilakukan di rumah kaca Laboratorium Hiijauan Makanan Ternak dan Pastura, Departemen Nutrisi dan Makanan Ternak, Fakultas Peternakan, Universitas Gadjah Mada. Perlakuan terdiri dari dosis colchicine  0,1?n lama perendaman colchicine  yaitu 6 jam, 12 jam dan 18 jam. Masing-masing perlakuan mendapatkan 12 kali pengulangan sehingga didapatkan 48 polybag. Data yang diamati dalam penelitian yaitu morfologi tanaman (tinggi tanaman, panjang tanaman, panjang daun, lebar daun, jumlah daun, diameter internode, panjang internode, jumlah tiller, warna daun dan warna batang) dan produksi biomasa (produksi segar, produksi bahan kering dan produksi bahan organik). Data morfologi dan produksi biomasa dianalisis menggunakan analisis variansi (ANNOVA) mengikuti rancangan percobaan pola searah dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT) jika terdapat perbedaan nyata pada perlakuan. Hasil penelitian menunjukkan bahwa morfologi dan produksi biomasa tanaman Brachiaria ruziziensis cv. Kennedy hasil perendaman menggunaan senyawa colchicine  menunjukkan perbedaan nyata (P<0>colchicine  selama 12 jam menunjukkan perbedaan nyata (P<0>internode, panjang internode dan jumlah tiller. Kesimpulan yang dapat diambil adalah perendaman selama 12 jam berpengaruh terhadap morfologi dan Brachiaria ruziziensis cv. Kennedy. Perendaman selama 6 dan 18 jam menurunkan morfologi dan produksi biomasa tanaman Brachiaria ruziziensis cv. Kennedy. 

The aim of this study was to evaluate the effect of colchicine  induction on morphological characteristic and biomass production of Ruzi grass (Brachiaria ruziziensis cv. Kennedy). This study was conducted in green house forage and pasture laboratory and forage pasture pasture laboratory. The dose of colchicine  treatment was 0,1% and soaking time of colchicine  were 6 hours, 12 hours, and 18 hours. Each treatment got 12 replication to get 48 polybags. The observed data were morphology (plant height, plant length, leaf length, leaf width, number of leaf, internode diameter, internode length, number of tiller, leaf colour and stem colour). Morphology and biomass production data were analysed using Analysis of Variance (ANNOVA) following one way pattern experimental design followed by Duncan’s Multiple Range Test if there were significantly different among the treatments. The results showed that colchicine  treatment had significant effect (P<0>0,05) on biomass production. Soaking time of 12 hours had significant effect (P<0>Brachiaria ruziziensis cv. Kennedy. Soaking time 12 hours of colchicine  treatment affected for Brachiaria ruziziensis cv. Kennedy to get biomass production and morphological character. Soaking time 6 hours and 18 hours can decrease morphology and biomass production of Brachiaria ruziziensis cv. Kennedy.

Kata Kunci : Brachiaria ruziziensis cv. Kennedy, morfologi, produksi biomasa, colcichine. /Brachiaria ruziziensis cv. Kennedy, morphology, biomass production, colchicine