Pengaruh Lama Sterilisasi Sinar Ultraviolet-C Pada Bahan Cetak Irreversible Hydrocolloid dan Polyvinyl Siloxane Terhadap Jumlah Staphylococcus Aureus
Gandis Harumi, Dr. drg. Sri Budi Barunawati, M.Kes, Sp.Pros.(K)
2023 | Tesis-Spesialis | S2 Prostodonsia
Hasil penelitian menunjukan jumlah Staphylococcus aureus terendah pada kelompok penyinaran 5 menit pada masing-masing jenis bahan: polyvinyl siloxane 4,34±0,63 x 105 CFU/ml, irreversible hydrocolloid 5,18±0,85 105 CFU/ml dan tertinggi pada kelompok kontrol pada masing-masing jenis bahan : irreversible hydrocolloid 8,58±0,57 x 105 CFU/ml, polyvinyl siloxane 7,14±0,55 x 105 CFU/ml. Hasil uji ANAVA dua jalur menunjukkan terdapat perbedaan bermakna antara pengaruh lama penyinaran sinar ultraviolet-C terhadap jumlah bakteri, terdapat perbedaan tidak bermakna antara irreversible hydrocolloid dan polyvinyl siloxane terhadap jumlah perlekatan bakteri (p>0,05). Lama sterilisasi ultraviolet-C selama 5 menit berpengaruh paling banyak menurunkan jumlah Staphylococcus aureus.
Irreversible hydrocolloid and polyvinyl siloxane are the impression materials most frequently used in dentistry for making replicas of tooth anatomy and oral cavity tissues. Staphylococcus aureus is a normal flora that can turn into a pathogen and cause various infections in the human body. The cross contamination can occur through the impression material from patients to operators and laboratory personel. Ultraviolet-C sterilization is one of option that can be used to sterilize impression materials. This study aims to examine the effect of Ultraviolet-C light sterilization duration on irreversible hydrocolloid and polyvinyl siloxane impression materials against the amount of Staphylococcus aureus.
Laboratory experimental research was carried out on 12 irreversible hydrocolloid subjects and 12 polyvinyl siloxane subjects (d=10 mm; l=15 mm) each of which was divided into 3 groups. The cylindrical impression material was contaminated in Staphylococcus aureus suspension according to McFarland 0.5 standard for 5 hours. The group of time Ultraviolet-C light sterilization; control, 3 minutes and 5 minutes. Sterilization using a Biosafety cabinet (Esco UV Lamp 30A), calculating the number of bacteria using a colony counter. The data obtained was analyzed use two-way ANOVA and post hoc LSD.
The results showed the lowest number of Staphylococcus aureus in the 5 minute irradiation group for each type of material: polyvinyl siloxane 4.34 ± 0.63 x 105 CFU/ml, irreversible hydrocolloid 5.18 ± 0.85 105 CFU/ml and the highest in control group for each type of material: irreversible hydrocolloid 8.58 ± 0.57 x 105 CFU/ml, polyvinyl siloxane 7.14 ± 0.55 x 105 CFU/ml. The results of the two-way ANOVA test showed that there was a significant difference in the effect of the length of UV-C light exposure on the number of bacteria (p>0.05), there was a non significant difference between irreversible and polyvinyl siloxane on the amount of bacterial attachment (p>0.05). The duration of ultraviolet-C sterilization for 5 minutes had the most effect on reducing the number of Staphylococcus aureus.
Kata Kunci : Irreversible hydrocolloid, polyvinyl siloxane, sinar ultraviolet-C, sterilisasi, Staphylococcus aureus
