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Deteksi Polimorfisme Mikro-InDel pada Gen PRL dan Cry2 serta Asosiasinya terhadap Fenotipe Litter Size Domba Sakub (Ovis aries)

Nazzun Assihhah, Prof. Dr. drh. Aris Haryanto, M.Si.

2023 | Skripsi | KEDOKTERAN HEWAN

Litter size merupakan indikator vital dalam penilaian kesuburan indukan pada sektor peternakan, termasuk komoditas domba (Ovis aries) Sakub. Keterbatasan pada seleksi konvensional fitur litter size dalam program breeding telah mempromosikan pendekatan alternatif molecular-assisted selection (MAS) yang dinilai lebih efektif. Berbagai studi mendemonstrasikan kegunaan polimorfisme insertion/deletion (InDel) gen kandidat seperti prolactin (PRL) dan cryptochrome circadian regulator 2 (Cry2) dalam seleksi litter size domba domestik. Penelitian terkait profil genotipik, parameter populasi, serta asosiasi InDel dengan litter size didasarkan pada implementasi markah InDel PRL dan Cry2 terhadap domba Sakub di Desa Pandansari dan Wanareja, Brebes, Jawa Tengah. Metode yang dilakukan meliputi amplifikasi lokus PRL-P1-ins-23 bp dan Cry2-P1-del-6 bp pada isolat DNA folikel rambut melalui touch-down polymerase chain reaction (TD-PCR) dilanjutkan elektroforesis gel agarose 3?n deteksi polimorfisme mikro-InDel. Analisis data populatif mengindikasikan indeks diversitas PRL (polimorfisme moderat) bergenotipe II, ID, dan DD yang konsisten dengan Hardy-Weinberg equilibrium (HWE), namun tidak dengan Cry2 (polimorfisme rendah) bergenotipe ID dan DD. Uji statistik ?2, ANOVA, dan t-test turut mengimplikasikan insignifikansi data asosiatif (p>0,05) antara InDel dengan partikularitas dan pluralitas litter size. Meski temuan ini tidak menunjukkan adanya kompetensi InDel PRL dan Cry2 sebagai penanda litter size domba Sakub, riset lanjutan masih diperlukan untuk mengonfirmasi data terkait.

Litter size is a vital indicator in evaluating the profitability and fecundity of broodstock in the livestock sector, including the Sakub sheep (Ovis aries) commodity. Limitations on conventional selection of litter size features in breeding programs have promoted molecular-assisted selection (MAS) as alternative approaches which are considered to be more effective. Various studies demonstrated the utility of InDel polymorphisms of candidate genes such as prolactin (PRL) and cryptochrome circadian regulator 2 (Cry2) in litter size selection of domestic sheep. In this study, the investigation of genotypic profiles, population parameters, and InDel associations with litter size was based on the implementation of PRL and Cry2 InDel markers on Sakub sheep in Pandansari and Wanareja Villages, Brebes, Central Java. The procedure involved amplification of the PRL-P1-ins-23 bp and Cry2-P1-del-6 bp loci in DNA isolates of hair follicle via touch-down polymerase chain reaction (TD-PCR) followed by 3% agarose gel electrophoresis and micro-InDel genotyping. Population data analysis indicated the diversity index of PRL (moderate polymorphism) that contains II, ID, and DD genotypes were consistent with Hardy-Weinberg equilibrium (HWE), but not with Cry2 (low polymorphism) that contains ID and DD genotypes. The ?2, t-test, and ANOVA statistical tests also implied insignificance (p>0.05) in associative data between InDel and particularity of litter sizes. Although these findings do not demonstrate the competence of InDel PRL and Cry2 as litter size markers for Sakub sheep, further research is still needed to confirm related data.

Kata Kunci : Domba Sakub, litter size, mikro-InDel, gen PRL, gen Cry2

  1. S1-2023-440020-abstract.pdf  
  2. S1-2023-440020-bibliography.pdf  
  3. S1-2023-440020-tableofcontent.pdf  
  4. S1-2023-440020-title.pdf