Pengaruh Perlakuan Colchicine terhadap Karakteristik Morfologi, Produksi Biomassa, dan Peningkatan Kualitas pada Rumput Rhodes (Chloris gayana cv. Callide))

Amarta Prameswari Purwasiwi, Prof. Ir. Nafiatul Umami, S.Pt., MP., Ph.D., IPM., ASEAN Eng

2023 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan kolkisin pada perendaman biji terhadap peningkatan karakteristik morfologi, pertumbuhan dan produksi biomassa pada rumput rhodes (Chloris gayana cv. Callide). Penelitian dilakukan di Lahan Hijauan Makanan Ternak dan Pastura, Fakultas Peternakan, Universitas Gadjah Mada dan Laboratorium Hijauan Makanan Ternak dan Pastura, Fakultas Peternakan, Universitas Gadjah Mada. Perlakuan pada biji Chloris gayana cv. Callide dengan kolkisin pada konsentrasi 0,1?ngan lama perendaman 2, 4, 6, dan tanpa perlakuan perendaman. Masing-masing perlakuan mendapatkan 12 kali pengulangan sehingga didapatkan 48 polybag. Parameter yang diamati dalam penelitian yaitu tinggi tanaman, panjang tanaman, panjang daun, lebar daun, jumlah daun, jumlah tiller, warna daun, warna batang, dan panjang internode), dan produksi biomassa meliputi produksi segar, produksi bahan kering, dan produksi bahan organik pada umur panen 60 hari. Data hasil penelitian dianalisis menggunakan ANOVA, dan perbedaan antar perlakuan diuji dengan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa pengaruh perendaman kolkisin (2 jam, 4 jam, dan 6 jam) pada konsentrasi 0,1?rpengaruh nyata (P<0>

This study aimed to determine the effect of colchicine treatment on  morphological characteristics, growth and biomass production on rhodes grass (Chloris gayana cv. Callide). The research was conducted at Green House Forage and Pasture Laboratory, and Forage and Pasture Laboratory, Faculty of Animal Sciences, Universitas Gadjah Mada. The seed treatment of Chloris gayana cv. Callide consisted of different soaking times of 2, 4, 6 hours, and the control with 0,1% colchicine solution. The treatment got 12 replication and there are 48 polybags. Parameters observed included plant height, plant length, leaf length, leaf width, number of leaves, number of tillers, leaf color, stem color, stem diameter, and internode length, and biomass production including fresh production, dry matter production, and organic matter production at 60 days after plants. Data was analyzed by ANOVA, and differences between treatments were tested with Duncan's Multiple Range Test (DMRT). The results showed that the effect of soaking with colchicine (2 hours, 4 hours, and 6 hours) at a concentration of 0.1% had a significant effect (P<0>colchicine gave higher yields on variables compare with no treatment. Colchicine immersion had no significant effect on plant length and internode length. The conclusion of the research was soaking for 2 hours increased leaf length, leaf width, and stem diameter. Soaking for 4 hours increased plant height, and soaking for 6 hours increased the number of leaves and fresh production, dry matter production, and organic matter production.

Kata Kunci : Chloris gayana cv. Callide, Colchicine, Mutasi, Poliploid, Chloris gayana cv. Callide, Colchicine, Mutation, Polyploid