Pengaruh Inkorporasi Injectable Platelet-Rich Fibrin dengan Nanofiber Kitosan dan Polyvinyl-Alcohol Terhadap Viabilitas Human Primary Fibroblast (Kajian In Vitro)
TASYA AMIRA, drg. Sri Pramestri Lastianny, M.S., Sp.Perio(K); 5. drg. Kwartarini Murdiastuti, Sp.Perio(K), Ph.D.
2023 | Tesis-Spesialis | SP PERIODONSIA
Penggunaan injectable Platelet-Rich
Fibrin (i-PRF) sebagai generasi ketiga konsentrasi platelet dari PRP
telah banyak digunakan sebagai salah satu perawatan regeneratif periodontal,
karena mengandung platelet, leukosit dan growth factors. Namun
kekurangan dari i-PRF adalah sifatnya yang cair, dan growth factors yang
mudah terdegradasi dengan cepat, sehingga diperlukan suatu scaffold atau
guided tissue regeneration (GTR) dalam bentuk nanofiber untuk dapat
meningkatkan masa pakai i-PRF. Nanofiber kitosan-polyvinyl alcohol salah
satu scaffold yang banyak diteliti dan dikembangkan karena bersifat
biokompatibel. Penelitian ini bertujuan untuk melihat viabilitas dari
inkorporasi i-PRF dengan nanofiber kitosan-polyvinyl alcohol, i-PRF serta
nanofiber kitosan-polyvinyl alcohol terhadap sel human primary
fibroblast.
Penelitian menggunakan nanofiber berukuran
diameter 5 mm yang direndam dalam i-PRF 5% selama 10 menit, kemudian dimasukkan
ke dalam sumuran yang berisi 5x103 sel/well. Setelah proses
inkubasi CO2 pada suhu 370 selama 24 jam, tiap sumuran
diberi Methilthiazol Tetrazolium (MTT) kemudian diinkubasi kembali selama
4 jam dan dilihat pada Multimode Microplate Reader. Viabilitas sel
diukur dengan menghitung presentase sel yang hidup. Data dianalisis menggunakan
uji One Way ANOVA.
Hasil penelitian menunjukkan inkorporasi
i-PRF/nanofiber kitosan-polyvinyl alcohol menunjukkan rerata
viabilitas sel diatas >90%. Hasil uji Post-Hoc menunjukkan tidak ada
perbedaan yang bermakna antara i-PRF/nanofiber kitosan-polyvinyl alcohol dengan
kelompok i-PRF (p>0,05). Kesimpulan dari penelitian ini adalah inkorporasi
i-PRF/ nanofiber kitosan-polyvinyl alcohol berpengaruh terhadap
viabilitas sel human primary fibroblast.
The use of injectable Platelet-Rich Fibrin (i-PRF) as a third generation of platelet
concentrations from PRP has been widely used as one of the regenerative
periodontal treatments, because it contains platelets, leukocytes and growth
factors. However, the drawbacks of i-PRF are its liquid nature and
growth factors which are easily degraded quickly, so a scaffold or guided
tissue radiation (GTR) in the form of nanofiber is needed to increase
the service life of i-PRF. Chitosan-polyvinyl alcohol nanofiber is one
of the scaffolds that has been extensively researched and developed because it
is biocompatible. This study aims to see the viability of i-PRF incorporation
with chitosan-polyvinyl alcohol nanofiber, i-PRF and chitosan-polyvinyl
alcohol nanofiber itself against human primary fibroblast cells.
This study used 5 mm-diameter nanofibers which were
soaked in 5% i-PRF for 10
minutes, then put into wells containing 5x103 cells/well. After the 24-hour
CO2 incubation process at 370, each well was treated with
Methylthiazole Tetrazolium (MTT) and then incubated for another 4 hours to
viewed on the Multimode Microplate Reader. Cell viability was measured by
calculating the percentage of viable cells. Data were analyzed using the One
Way ANOVA test.
The
results showed that the incorporation of i-PRF/chitosan-polyvinyl alcohol nanofiber
showed an average cell viability of >90%. Post-Hoc test results showed no
significant difference between the i-PRF/chitosan-polyvinyl alcohol
nanofiber and the i-PRF group (p>0,05). The conclusion of this study
is that the incorporation of i-PRF/ chitosan-polyvinyl alcohol
nanofibers has an effect on the viability of human primary fibroblast cells.
Kata Kunci : Injectable Platelet-Rich Fibrin, nanofiber kitosan-polyvinyl alcohol¬, viabilitas, biokompatibel, sitotoksisitas, periodontitis