Pengaruh Inkorporasi Injectable Platelet-Rich Fibrin dengan Nanofiber Kitosan dan Polyvinyl-Alcohol Terhadap Viabilitas Human Primary Fibroblast (Kajian In Vitro)

TASYA AMIRA, drg. Sri Pramestri Lastianny, M.S., Sp.Perio(K); 5. drg. Kwartarini Murdiastuti, Sp.Perio(K), Ph.D.

2023 | Tesis-Spesialis | SP PERIODONSIA

Penggunaan injectable Platelet-Rich Fibrin (i-PRF) sebagai generasi ketiga konsentrasi platelet dari PRP telah banyak digunakan sebagai salah satu perawatan regeneratif periodontal, karena mengandung platelet, leukosit dan growth factors. Namun kekurangan dari i-PRF adalah sifatnya yang cair, dan growth factors yang mudah terdegradasi dengan cepat, sehingga diperlukan suatu scaffold atau guided tissue regeneration (GTR) dalam bentuk nanofiber untuk dapat meningkatkan masa pakai i-PRF. Nanofiber kitosan-polyvinyl alcohol salah satu scaffold yang banyak diteliti dan dikembangkan karena bersifat biokompatibel. Penelitian ini bertujuan untuk melihat viabilitas dari inkorporasi i-PRF dengan nanofiber kitosan-polyvinyl alcohol, i-PRF serta nanofiber kitosan-polyvinyl alcohol terhadap sel human primary fibroblast.

Penelitian menggunakan nanofiber berukuran diameter 5 mm yang direndam dalam i-PRF 5% selama 10 menit, kemudian dimasukkan ke dalam sumuran yang berisi 5x10³ sel/well. Setelah proses inkubasi CO₂ pada suhu 37⁰ selama 24 jam, tiap sumuran diberi Methilthiazol Tetrazolium (MTT) kemudian diinkubasi kembali selama 4 jam dan dilihat pada Multimode Microplate Reader. Viabilitas sel diukur dengan menghitung presentase sel yang hidup. Data dianalisis menggunakan uji One Way ANOVA.

Hasil penelitian menunjukkan inkorporasi i-PRF/nanofiber kitosan-polyvinyl alcohol menunjukkan rerata viabilitas sel diatas >90%. Hasil uji Post-Hoc menunjukkan tidak ada perbedaan yang bermakna antara i-PRF/nanofiber kitosan-polyvinyl alcohol dengan kelompok i-PRF (p>0,05). Kesimpulan dari penelitian ini adalah inkorporasi i-PRF/ nanofiber kitosan-polyvinyl alcohol berpengaruh terhadap viabilitas sel human primary fibroblast.

The use of injectable Platelet-Rich Fibrin (i-PRF) as a third generation of platelet concentrations from PRP has been widely used as one of the regenerative periodontal treatments, because it contains platelets, leukocytes and growth factors. However, the drawbacks of i-PRF are its liquid nature and growth factors which are easily degraded quickly, so a scaffold or guided tissue radiation (GTR) in the form of nanofiber is needed to increase the service life of i-PRF. Chitosan-polyvinyl alcohol nanofiber is one of the scaffolds that has been extensively researched and developed because it is biocompatible. This study aims to see the viability of i-PRF incorporation with chitosan-polyvinyl alcohol nanofiber, i-PRF and chitosan-polyvinyl alcohol nanofiber itself against human primary fibroblast cells.

This study used 5 mm-diameter nanofibers which were soaked in 5% i-PRF for 10 minutes, then put into wells containing 5x10³ cells/well. After the 24-hour CO₂ incubation process at 37⁰, each well was treated with Methylthiazole Tetrazolium (MTT) and then incubated for another 4 hours to viewed on the Multimode Microplate Reader. Cell viability was measured by calculating the percentage of viable cells. Data were analyzed using the One Way ANOVA test.

The results showed that the incorporation of i-PRF/chitosan-polyvinyl alcohol nanofiber showed an average cell viability of >90%. Post-Hoc test results showed no significant difference between the i-PRF/chitosan-polyvinyl alcohol nanofiber and the i-PRF group (p>0,05). The conclusion of this study is that the incorporation of i-PRF/ chitosan-polyvinyl alcohol nanofibers has an effect on the viability of human primary fibroblast cells.

Kata Kunci : Injectable Platelet-Rich Fibrin, nanofiber kitosan-polyvinyl alcohol¬, viabilitas, biokompatibel, sitotoksisitas, periodontitis