PENAMBAHAN NANOPARTIKEL TEMBAGA OKSIDA PADA MINERAL TRIOKSIDA AGREGAT PUTIH UNTUK MENINGKATKAN KUAT TEKAN DAN SIFAT ANTIBAKTERI STREPTOCOCCUS MUTANS DAN ESCHERICHIA COLI

RONY IRAWAN, Prof. Dr.rer.nat. Nuryono, M.S.; Prof. Drs. Sri Juari Santosa, M.Eng., Ph.D.

2023 | Skripsi | S1 KIMIA

Sintesis nanopartikel tembaga oksida (CuONP) untuk meningkatkan sifat mekanik dan antibakteri pada mineral trioksida agregat putih (WMTA) telah berhasil dilakukan. Tujuan penelitian ini adalah sintesis dan karakterisasi CuONP, serta mempelajari efek penambahan CuONP terhadap sifat fisik (kekristalan dan kuat tekan) dan sifat antibakteri WMTA terhadap bakteri Streptococcus mutans (S. mutans) dan Escherichia coli (E. coli). Nanopartikel CuO disintesis dengan mencampurkan CuSO4�·5H2O 0,5 M dan Na�¬2CO3 0,5 M pada variasi volume 15, 25 dan 42 mL melalui metode dekomposisi termal dan presipitasi. Material WMTA-CuO dibuat dengan mencampurkan WMTA dan CuONP pada variasi persentase massa (1, 2, dan 3 %). Nanopartikel CuO, material WMTA, dan WMTA-CuO dikarakterisasi dengan Fourier Transform Infrared (FTIR), X-Ray Diffractometer (XRD), dan Scanning Electron Microscopy-Energy Dispersive Xray (SEM-EDX). Analisis sifat mekanik WMTA dikaji dengan penambahan CuONP pada WMTA melalui pengujian kuat tekan dan pengujian sifat antibakteri terhadap bakteri S. mutans dan E. coli dengan waktu pendiaman pelet WMTA-CuO selama 14 hari. Hasil karakterisasi FTIR dan XRD menunjukkan bahwa CuONP telah berhasil disintesis dan diperoleh ukuran rerata kristal sebesar 25,03 nm, ditandai adanya puncak-puncak karakteristik CuO dengan ukuran kristal pada masing-masing variasi, yaitu 28,41; 18,79; dan 27,90 nm. Penambahan CuONP pada WMTA mampu meningkatkan sifat mekaniknya, yaitu kuat tekan tertinggi 10,86�±0,78 MPa ditunjukkan pada WMTA yang ditambah CuONP 1 % (WMTA-CuO(1)). Penambahan CuONP memberikan peningkatan terhadap antibakteri S. mutans dengan nilai zona hambat sebesar 15,42�±0,54 mm untuk WMTA-CuO(3) dan terhadap E. coli dengan nilai zona hambat sebesar 16,70�±0,62 mm pada WMTA-CuO(1).

The synthesis of copper oxide nanoparticles (CuONP) to improve the mechanical and antibacterial properties of white mineral trioxide aggregate (WMTA) has been successfully carried out. The objectives of this research were the synthesis and characterization of CuONP, as well as studying the effect of CuONP addition on the physical properties (crystallinity and compressive strength) and antibacterial properties of WMTA against Streptococcus mutans (S. mutans) and Escherichia coli (E. coli) bacteria. CuO nanoparticles were synthesized by mixing CuSO4�·5H2O 0.5 M and Na2CO3 0.5 M at various volumes of 15, 25 and 42 mL through thermal decomposition and precipitation method. WMTA-CuO material was prepared by mixing WMTA and CuONP at various mass percentages (1, 2, and 3 %). CuO nanoparticles, WMTA, and WMTA-CuO materials were characterized by Fourier Transform Infrared (FTIR), X-Ray Diffractometer (XRD), and Scanning Electron Microscopy-Energy Dispersive Xray (SEM-EDX). The mechanical properties of WMTA were analyzed with the addition of CuONP to WMTA through compressive strength testing and antibacterial properties testing against S. mutans and E. coli bacteria with 14 days of WMTA-CuO pellet incubation time. The results of FTIR and XRD characterization show that CuONP has been successfully synthesized and obtained an average crystal size of 25.03 nm, marked by the presence of characteristic peaks of CuO with crystal size in each variation, namely 28.41; 18.79; and 27.90 nm. The addition of CuONP to WMTA was able to improve its mechanical properties, namely the highest compressive strength of 10.86�±0.78 MPa shown in WMTA added with 1% CuONP (WMTA-CuO(1)). The addition of CuONP improved the antibacterial effect against S. mutans with an inhibition zone value of 15.42�±0.54 mm for WMTA-CuO(3) and against E. coli with an inhibition zone value of 16.70�±0.62 mm for WMTA-CuO(1).

Kata Kunci : antibakteri, nanopartikel CuO, sifat mekanik, WMTA