Degradasi Bahan Organik dan Reduksi Amonia Limbah Cair Rumah Potong Ayam (RPA) Oleh Pseudomonas sp. LS3K

CHARISMA RIZQI A, Ir. Nanung Agus Fitriyanto, S.Pt., M.Sc., Ph.D., IPM

2023 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pertumbuhan Pseudomonas sp. LS3K dalam medium yang mengandung limbah cair Rumah Potong Ayam (RPA) dan kemampuannya untuk menurunkan kadar amonia dan kadar bahan organik limbah dengan menjadi agen bioremediasi serta biotransformasi. Penelitian dilakukan dengan menumbuhkan bakteri Pseudomonas sp. LS3K pada medium cair limbah RPA dengan mengatur medium tersebut di pH 7 kemudian menggunakan konsentrasi limbah cair RPA sebesar 0%, 25%, 50%, 75%, dan 100%. Berdasarkan perlakuan tersebut kemudian dilihat kemampuan Pseudomonas sp. LS3K untuk dapat mereduksi ammonia dan bahan organik yang terdapat pada limbah cair RPA. Variabel yang diamati yaitu pertumbuhan bakteri, konsentrasi amonia, nilai Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Solid (TS), dan Total Suspended Solid (TSS). Setiap perlakuan dilakukan dengan replikasi sebanyak tiga kali. Data hasil penelitian dianalisis secara statistik menggunakan variansi Rancangan Acak Lengkap (RAL) pola searah dan dilanjutkan dengan uji beda Duncans Multiple Range Test (DMRT). Hasil yang diperoleh menunjukkan bahwa Pseudomonas sp. LS3K dapat tumbuh di medium cair dan padat pada setiap perlakuan. Persentase penurunan terbesar nilai TS yaitu 56,12 plus minus 8,00%, TVS 77,93 plus minus 9,54%, TDS 47,30 plus minus 2,60%, TSS 84,27 plus minus 15,40%, BOD 88,90 plus minus 13,59%, COD 59,76 plus minus 18,07%, untuk kadar ammonia yaitu 71,69 plus minus 17,11% pada P0. Pseudomonas sp. LS3K dapat menurunkan kadar ammonia dan bahan organic limbah cair RPA.

The aim of this research was to determine the growth of Pseudomonas sp. LS3K in the media that contain chicken slaughterhouse wastewater and its ability to reduce ammonia and organic matter levels as a bioremediation and biotransformation agent. The research was conducted by growing the Pseudomonas sp. LS3K in a liquid and solid media that contain chicken slaughterhouse wastewater at 0%, 25%, 50%, 75%, and 100%. Bacterial viability was also observed by inoculating bacteria that had been incubated in a liquid waste media for 24 and 48 hours in a solid nutrien medium. The ability of Pseudomonas sp. LS3K to reduce ammonia and organic matter contained in wastewater was tested before and after the bioremediation by aeration process for 4 days. This research consisted of 5 treatments, which were P0 100% waste without bacteria, P1 25% waste with 2% bacteria, P2 50% waste with 2% bacteria, P3 75% waste with 2% bacteria, and P4 100% waste with 2% bacteria. The data obtained included bacterial growth, bacterial viability, the value of Total Solid (TS), Total Volatile Solid (TVS), Total Suspended Solid (TSS), Total Dissolved Solid (TDS), Biological Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), and ammonia level. Each treatment consisted of three replications. The research data were analyzed statistically using analysis of variance with unidirectional patterns of Complete Randomized Design (CRD) and continued with Duncan's Multiple Range Test (DMRT). The results showed that Pseudomonas sp. LS3K could grow in both liquid and solid media for each treatment. The largest percentage reduction for the TS value was 56,12plus minus 8,00%, TVS 77,93 plus minus 9,54%, TDS 47,30plus minus 2,60%, TSS 84,27 plus minus 15,40%, BOD 88,90 plus minus 13,59%, COD 59,76 plus minus 18,07%, for ammonia level was 71,69 plus minus 17,11% pada in P4. Pseudomonas sp. LS3K could reduce ammonia and organic matter levels in chicken slaughterhouse wastewater.

Kata Kunci : limbah cair, rumah potong ayam, Pseudomonas sp. LS3K, nitrifikasi, bioremediasi