Isolasi, Identifikasi Molekuler, dan Deteksi Gen Virulen Bakteri Edwardsiella tarda yang Diisolasi dari Lele Dumbo (Clarias sp.) di Daerah Istimewa Yogyakarta

MAULANA ALIYUDDIN KHOIRY, Dr. Ir. Triyanto, M.Si.

2023 | Skripsi | S1 AKUAKULTUR

Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi serta mendeteksi keberadaan gen virulen (fimA, katB dan esrB) bakteri Edwardsiella tarda dari usus lele dumbo (Clarias sp.) di Daerah Istimewa Yogyakarta. Bakteri E. tarda diisolasi dari usus lele dumbo dan diinokulasikan pada medium SSA dan MCA. Isolat bakteri yang mempunyai koloni dengan morfologi bentuk circular, elevasi convex, tepian entire, opsitas opaque, translucent dengan pigmen bening dan terdapat titik hitam di pusat koloni pada SSA dan pigmen bening pada MCA, selanjutnya dilakukan uji presumtif meliputi uji katalase, oksidase dan pengamatan Gram. Isolat-isolat yang diduga kuat sebagai E. tarda dilanjutkan amplifikasi dengan primer spesifik (etfD-F etfD-R). Isolat bakteri E. tarda yang diperoleh selanjutnya diamplifikasi gen virulennya meliputi gen fimA, katB, dan esrB. Penelitian ini berhasil mendapatkan sebanyak 18 isolat bakteri E.tarda dari 17 ekor lele dumbo di Daerah Istimewa Yogyakarta. Hasil penelitian menunjukkan terdapat 5 isolat yang mempunyai gen fimA, 4 isolat mempunyai gen katB dan 4 isolat mempunyai gen esrB, namun hanya terdapat satu isolat yang terdeteksi mempunyai ketiga gen virulen tersebut.

The aim of the present study was to isolate, identify, and detect the virulence genes of Edwardsiella tarda bacteria (fimA, katB, and esrB) isolated from the intestinal tract of catfish (Clarias sp.) from several catfish farms in Special Region of Yogyakarta. The bacteria were isolated from catfish and inoculated on both SSA and MCA media. Bacterial isolates that had colonies with circular morphology, convex elevation, entire margin, opaque opacity, translucent with clear pigment and a black dot in the centre of the colony on SSA and clear pigment on MCA were then carried out by presumptive tests including catalase, oxidase and Gram's observation. The isolates strongly suspected of being E. tarda were amplified using specific primers (etfD-F etfD-R). E. tarda isolates were then amplified with three virulence gene primers (fimA, katB and esrB). In this study, 18 isolates of E. tarda bacteria were obtained from 17 catfish in SR Yogyakarta. The results showed that there were 5 isolates that had the fimA gene, 4 isolates had the katB gene, and 4 isolates had the esrB gene, but only one isolate was detected to have the three virulence genes.

Kata Kunci : Daerah Istimewa Yogyakarta, Edwardsiella tarda, gen virulen, lele dumbo