Ekspresi Relatif mRNA p53, Apaf-1 dan Survivin terkait Apoptosis serta NF-kappaB, TSP-1 dan bFGF terkait Angiogenesis pada Jaringan Tumor Payudara Tikus Pasca Electro Capacitive Cancer Therapy Frekuensi 150 kHz

NURUL HIDAYAH, Dr.Rarastoeti Pratiwi, M.Sc.; Assoc. Dr.dr.Agung Putra, M.Si.Med.

2023 | Tesis | MAGISTER BIOTEKNOLOGI

Latar belakang: Kanker payudara merupakan kanker yang paling banyak menyebabkan kematian pada wanita di dunia. Perkembangan kanker difasilitasi oleh inhibisi apoptosis serta induksi angiogenesis. Terapi kanker saat ini masih dijumpai masalah berupa kekambuhan, resistensi dan efek samping. Terapi medan listrik statis non-contact, Electro Capacitive Cancer Therapy (ECCT) dengan frekuensi menengah merupakan koterapi yang dikembangkan untuk menghambat proliferasi sel-sel tumor. Tujuan: Penelitian ini bertujuan untuk mengetahui ekspresi mRNA p53, Apaf-1, Survivin terkait apoptosis dan NF-κB, bFGF dan TSP-1 terkait angiogenesis pada jaringan tumor payudara tikus pasca ECCT frekuensi 150 kHz. Metode: Sampel jaringan payudara dan nodul tumor payudara tikus yang tersimpan dalam RNA later pada suhu -20°C. Jaringan tersebut berasal dari kelompok Non Induksi Non Terapi (NINT); Induksi Non Terapi (INT); Non Induksi Terapi (NIT) serta Induksi Terapi IT). Ekspresi mRNA p53, Apaf-1, NF-κB, bFGF dan TSP-1 dianalisis menggunakan qRT-PCR dan dihitung dengan rumus Livak. Data dianalisis menggunakan One-way ANOVA dan Post-hoc LSD. Hasil: Ekspresi mRNA p53, Apaf-1 dan TSP-1 pada kelompok IT meningkat secara signifikan, serta ekspresi mRNA Survivin dan bFGF menurun secara signifikan dibandingkan kelompok INT. Ekspresi mRNA NF-κB pada kelompok IT tetap sama dengan kelompok INT. Kesimpulan: ECCT frekuensi 150 kHz dapat mengupregulasi ekspresi mRNA p53, Apaf-1 dan TSP-1 serta mendownregulasi ekspresi mRNA Survivin dan bFGF tetapi tidak berpengaruh pada ekspresi mRNA NF-κB pada jaringan tumor payudara tikus.

ABSTRACT Background: Breast cancer is the most common cancer that causes death in women worldwide. Cancer development is facilitated by the inhibition of apoptosis and the induction of angiogenesis. Current cancer therapy still encounters problems with recurrence, resistance, and side effects. Non-contact static electric field therapy, Electro Capacitive Cancer Therapy (ECCT) with medium frequency is a therapy developed to inhibit the proliferation of tumor cells. Objective: This study aims to determine the mRNA expression of p53, Apaf-1, and Survivin related to apoptosis and NF-κB, bFGF and TSP-1 related to angiogenesis in rat breast tumor tissue after ECCT frequency of 150 kHz. Methods: Breast tissue samples and rat breast tumor nodules stored in RNA later at -20°C. The tissue comes from the Non-Induction Non-Therapy (NINT) group; Induction Non-Therapy (INT); Non-Induction Therapy (NIT); Induction and Therapy (IT). mRNA expression of p53, Apaf-1, NF-κB, bFGF and TSP-1 were analyzed using qRT-PCR and calculated with the Livak formula. Data were analyzed using One-way ANOVA and post-hoc LSD. Results: mRNA expression of p53, Apaf-1 and TSP-1 in the IT group increased significantly, and mRNA expression of Survivin and bFGF decreased significantly compared to the INT group. But the expression of NF-κB mRNA in the IT group remained the same as in the INT group. Conclusion: ECCT with a frequency of 150 kHz could upregulate p53, Apaf-1 and TSP-1 mRNA expression and downregulate Survivin and bFGF mRNA expression but had no effect on NF-κB mRNA expression in rat breast tumor tissue.

Kata Kunci : Kanker payudara, ECCT, Apoptosis, Angiogenesis, Ekspresi Gen