KEMAMPUAN Bacillus cereus TD5B DALAM MENURUNKAN KADAR AMONIA DAN MENDEGRADASI BAHAN ORGANIK LIMBAH CAIR RUMAH POTONG AYAM

RIANA TRISNANINGSIH, Ir. Nanung Agus Fitriyanto, S.Pt., M.Sc., Ph.D., IPM.; Prof. Ir. Yuny Erwanto, S.Pt., M.P., Ph.D., IPM.

2023 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pertumbuhan Bacillus cereus TD5B dalam medium yang mengandung limbah cair RPA dan kemampuannya untuk menurunkan kadar amonia dan bahan organik limbah dengan menjadi agen bioremediasi. Penelitian dilakukan dengan menumbuhkan Bacillus cereus TD5B pada medium cair dan padat dengan konsentrasi limbah cair RPA sebesar 0%, 25%, 50%, 75%, dan 100%. Viabilitas bakteri juga dilihat dengan menginokulasikan bakteri yang telah diinkubasi dalam medium limbah cair selama 24 dan 48 jam pada medium nutrien padat. Kemampuan Bacillus cereus TD5B untuk mereduksi amonia dan bahan organik limbah cair RPA diuji sebelum dan sesudah proses aerasi selama 4 hari. Perlakuan yang diberikan sama dengan uji pertumbuhan bakteri dengan perbedaan pada P0 yaitu 100% limbah tanpa penambahan bakteri dan direplikasi sebanyak 3 kali. Data yang diperoleh yaitu pertumbuhan bakteri, viabilitas bakteri, nilai total solid (TS), total volatile solid (TVS), total suspended solid (TSS), total dissolved solid (TDS), biological oxygen demand (BOD5), chemical oxygen demand (COD), dan kadar amonia. Data hasil penelitian dianalisis secara statistik menggunakan variansi rancangan acak lengkap (RAL) pola searah dan dilanjutkan dengan Duncan's multiple range test (DMRT). Hasil yang diperoleh menunjukkan bahwa Bacillus cereus TD5B dapat tumbuh di medium cair dan padat pada setiap perlakuan. Persentase penurunan terbesar untuk nilai TS yaitu 51,10 ± 6,48% pada P2, nilai TVS yaitu 77,20 ± 11,99% pada P2, nilai TSS yaitu 88,49 ± 4,51% pada P4, nilai TDS yaitu 48,62 ± 5,34% pada P1, nilai BOD5 yaitu 95,81 ± 3,19% pada P0, nilai COD yaitu 61,71 ± 21,20% pada P0, dan untuk kadar amonia yaitu sebesar 63,38 ± 21,48% pada P4. Bacillus cereus TD5B dapat menurunkan kadar amonia dan bahan organik limbah cair RPA.

The aim of this research was to determine the growth of Bacillus cereus TD5B in the media that contain chicken slaughterhouse wastewater and its ability to reduce ammonia and organic matter levels as a bioremediation agent. The research was conducted by growing the Bacillus cereus TD5B in a liquid and solid media that contain chicken slaughterhouse wastewater at 0%, 25%, 50%, 75%, and 100%. Bacterial viability was also observed by inoculating bacteria that had been incubated in a liquid waste media for 24 and 48 hours in a solid nutrient medium. The ability of Bacillus cereus TD5B to reduce ammonia and organic matter contained in wastewater was tested before and after the bioremediation by aeration process for 4 days. The treatments given were the same as the bacterial growth test with a difference in P0 which was 100% waste without bacteria. The data obtained included bacterial growth, bacterial viability, the value of total solid (TS), total volatile solid (TVS), total suspended solid (TSS), total dissolved solid (TDS), biological oxygen demand (BOD5), chemical oxygen demand (COD), and ammonia level. Each treatment consisted of three replications. The research data were analyzed statistically using analysis of variance with unidirectional patterns of complete randomized design (CRD) and continued with Duncan's multiple range test (DMRT). The results showed that Bacillus cereus TD5B could grow in both liquid and solid media for each treatment. The largest percentage reduction for the TS value was 51.10 ± 6.48% in P2, the TVS value was 77.20 ± 11.99% in P2, the TSS value was 88.49 ± 4.51% in P4, the TDS value was 48.62 ± 5.34% in P3, the BOD5 value was 95.81 ± 3.19% in P0, the COD value was 61.71 ± 21.20% in P0, and for ammonia level was 63.38 ± 21.48% in P4. Bacillus cereus TD5B could reduce ammonia and organic matter levels in chicken slaughterhouse wastewater.

Kata Kunci : limbah cair, rumah potong ayam, Bacillus cereus, bioremediasi