TINGKAT KERUSAKAN DNA SPERMATOZOA SAPI BALI DAN SAPI MADURA PASCA THAWING

WISNU OKA PURWANTORO, Prof. Ir. Diah Tri Widayati, M.P., Ph.D., IPM. ; Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng.

2022 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui tingkat kerusakan DNA spermatozoa sapi Bali dan sapi Madura pasca thawing. Penelitian ini dilakukan di Laboratorium Fisiologi dan Reproduksi Ternak, Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta. Delapan belas straw semen beku dari sapi Bali dan sapi Madura digunakan dalam penelitian ini. Data yang diamati yaitu motilitas, abnormalitas, dan kerusakan DNA spermatozoa. Motilitas diamati berdasarkan gerakan individu spermatozoa dan abnormalitas diamati berdasarkan abnormal bentuk kepala dan ekor spermatozoa. Pengujian kerusakan DNA dilakukan dengan penambahan Low Melting Point Agarose (LMP-agarose) 0,8 persen, Lisys Solution (LS) II, pewarnaan eosin dan diamati menggunakan mikroskop perbesaran 400 kali. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa rata-rata motilitas, abnormalitas dan kerusakan DNA spermatozoa sapi Bali pasca thawing secara berturut-turut yaitu 56,94 kurang lebih 3,89 persen, 13,06 kurang lebih 3,33 persen dan 0,55 kurang lebih 0,35 persen. Rata-rata motilitas, abnormalitas dan kerusakan DNA spermatozoa sapi Madura berturut-turut yaitu 54,72 kurang lebih 3,63 persen, 13 kurang lebih 3,74 persen, dan 0,62 kurang lebih 0,35 persen. Berdasarkan hasil penelitian yang dilakukan dapat disimpulkan bahwa bahwa motilitas, abnormalitas berada pada kisaran normal dan kerusakan DNA spermatozoa sapi Bali dan sapi Madura masih berada di bawah nilai standar DNA Fragmentasi Indeks (DFI).

The purpose of this study was to investigate the level of spermatozoa DNA damage of Bali bulls and Madura bulls after thawing. This research carried out at Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta. Eighteen frozen semen straws from Bali and Madura bulls were use in this research. The data observed were motility, abnormality and spermatozoa DNA damage. Motility was observed based on individual movements of spermatozoa and abnormality observed based on abnormal head and tail of spermatozoa shape. DNA damage testing was carried out by adding 0,8 percent Low Melting Point Agarose (LMP-Agarose), Lisys Solution II, stained with eosin and observed using a microscope with a magnification of 400. The data obtained were descriptive analyzed. The results showed that the average motility, abnormality, and DNA damage of spermatozoa of Bali bulls after thawing were 56.94 more or less 3.89 percent, 13.06 more or less 3.33 percent, and 0.55 more or less 0.35 percent, respectively. The average motility, abnormality, and DNA damage of Madura bull�s spermatozoa were 54.72 more or less 3.63 percent, 13 more or less 3.74 percent, and 0.62 more or less 0.35 percent, respectively. Based on results of this research it is known that the motility and abnormality were in the normal range. Spermatozoa DNA damage Bali bull and Madura bull were still below the standard value of DNA Fragmentation Index (DFI).

Kata Kunci : Kerusakan DNA spermatozoa, pasca thawing, sapi Bali, sapi Madura