CEMARAN BAKTERI PADA UDANG (Liptopenaeus vannamei) YANG DIJUAL DI PASAR SOROBAYAN KABUPATEN BANTUL

MILA MELISA W, Dr. drh. Widagdo Sri Nugroho, M.P.

2022 | Skripsi | S1 KEDOKTERAN HEWAN

Berdasarkan data Statistik Kementrian Kelautan dan Perikanan Daerah Istimewa Yogyakarta produksi udang dari tahun ketahun mengalami peningkatan. Seiring dengan meningkatnya produksi udang di Daerah Istimewa Yogyakarta tentu perlu didukung dengan tersedianya produk yang aman dan sehat untuk dikonsumsi masyarakat. Penelitian ini bertujuan untuk mengidentifikasi cemaran bakteri meliputi Total Plate Count (TPC), Salmonella sp. dan Escherichia coli pada udang yang berasal dari Pasar Sorobayan Kabupaten Bantul. Sebanyak 10 unit sampel (@10 gram) udang yang didapatkan dari pasar tradisional Sorobayan Kabupaten Bantul. Sampel udang diperiksa terhadap Total Plate Count (TPC), isolasi dan identifikasi Salmonella sp. dan Escherichia coli. Perhitungan TPC menggunakan media Plate Count Agar (PCA), isolasi Salmonella sp. menggunakan Xylose Lysine Dextrose Agar (XLD) dan isolasi Escherichia coli menggunakan Eosin Methylen Blue Agar (EMB). Hasil isolasi dan identifikasi dianalisis secara deskriptif. Hasil penelitian ini menunjukkan bahwa Total Plate Count (TPC) sebanyak 2,2 x 106 CFU/gr, cemaran Escherichia coli sebesar 40% namun tidak ditemukan cemaran Salmonella sp. Cemaran mikroba pada udang yang dijual di Pasar Sorobayan Kabupaten Bantul melebihi standar yang ditentukan dan masih ditemukan tercemar E. coli.

Based on the Ministry of Maritime Affairs and Fisheries statistics, the production of shrimp in Daerah Istimewa Yogyakarta is increasing annually. The quantity improvement of shrimp production in D.I. Yogyakarta needs to be supported with products that are safe and healthy for public consumption. This study aims to identify bacterial contamination including Total Plate Count (TPC), Salmonellaa sp., and Escherichia coli in shrimp that are sold at Sorobayan Market Bantul Regency. A total of 10 sample units (@10 gram) of shrimp were obtained f rom the Sorobayan traditional markets. Sampel units were used for inspection of the Total Place Count (TPC), Salmonella sp. and Escherichia coli isolation identification. The method used in research were Plate Count Agar (PCA) for TPC, Xylose Lysine Dextrose Agar (XLD) for Salmonella sp. isolation and Eosin Methylene Blue Agar (EMB) for Escherichia coli isolation and identification data results were analyzed decriptively. The results of this study showed that quantification of shrimp Total Plate Count (TPC) is 2,2 x 106 CFU/gr, a total of 40% positive results for Escherichia coli isolation and negative results for Salmonella sp. Microbial contamination of shrimp sold at Sorobayan market, Bantul regency exceeded the specified standard and was still found to be contaminated with Escherichia coli.

Kata Kunci : Shrimp, Total Plate Count (TPC), Salmonella sp., Escherichia coli