PENGARUH SUPLEMENTASI ARGAN OIL, ROSEMARY OIL, DAN PEPPERMINT OIL DALAM MEDIUM PENCUCIAN SPERMA SETELAH THAWING TERHADAP KUALITAS SPERMATOZOA SAPI SIMMENTAL

BRAHMANTARI CHRISTA ARINDITA, Widya Asmarawati, S.Pt., M.Sc.

2022 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi argan oil, rosemary oil, dan peppermint oil pada medium pencucian sperma setelah thawing terhadap kualitas spermatozoa sapi Simmental. Penelitian dilakukan di Laboratorium Fisiologi dan Reproduksi Ternak Fakultas Peternakan Universitas Gadjah Mada. Materi yang digunakan dalam penelitian ini adalah 40 straw sperma beku sapi Simmental yang berasal dari sapi berumur antara 4 sampai 6 tahun produksi UPTD BPBPTDK Dinas Pertanian DIY. Sperma beku di-thawing pada suhu 37 derajat Celcius selama 30 detik dan dicuci dengan metode direct swim up dengan medium skim base extender (SBE) dan ditambah suplementasi argan oil (AO), rosemary oil (RO), dan peppermint oil (PO). Pengujian kualitas spermatozoa setelah thawing terdiri dari 8 perlakuan yang berbeda yaitu SBE (kontrol), SBE + AR 1 persen, SBE + RO 1 persen, SBE + PO 1 persen, SBE + AR 1 persen + RO 1 persen, SBE + AR 1 persen + PO 1 persen, SBE + RO 1 persen + PO 1 persen, dan SBE + AR 1 persen + RO 1 persen + PO 1 persen. Data yang diperoleh berupa motilitas, viabilitas, abnormalitas, keutuhan tudung akrosom dan integritas membran spermatozoa dianalisis dengan menggunakan uji Kruskal-Wallis. Hasil analisis data menunjukkan terdapat perbedaan yang signifikan terhadap motilitas, viabilitas, dan integritas membran (P kurang dari 0,05). Motilitas dan viabilitas tertinggi yaitu perlakuan penambahan SBE saja masing-masing 29,38 kurang lebih 9,80 persen dan 68,13 kurang lebih 6,81 persen. Integritas membran tertinggi pada perlakuan SBE + AR 1 persen + RO 1 persen + PO 1 persen yaitu 80,00 kurang lebih 6,23 persen. Kesimpulan dari penelitian ini adalah perlakuan penambahan SBE saja memiliki pengaruh paling baik terhadap motilitas dan viabilitas, sedangkan perlakuan penambahan AR 1 persen, ROS 1 persen, dan PO 1 persen paling baik terhadap integritas membran.

This study aims to determine the effect of argan oil, rosemary oil, and peppermint oil supplementation on sperm washing medium after thawing on the sperm quality of Simmental cattle. The research was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Husbandry, Gadjah Mada University. The material used in this study was 40 straws of Simmental bull frozen sperm from 4 to 6 years produced by UPTD BPBPTDK DIY Agriculture Service. Frozen sperm were thawed at 37 degree Celcius for 30 seconds and washed by direct swim up method with medium skim base extender (SBE) and supplemented with argan oil (AO), rosemary oil (RO), and peppermint oil (PO). Spermatozoa quality testing after thawing consisted of 8 different treatments, namely SBE (control), SBE+AR 1 percent, SBE+RO 1 percent, SBE + PO 1 percent, SBE + AR 1 percent + RO 1 percent, SBE + AR 1 percent + PO 1 percent, SBE + RO 1 percent + PO 1 percent, and SBE + AR 1 percent + RO 1 percent + PO 1 percent. The data obtained in the form of motility, viability, abnormalities, acrosome integrity and spermatozoa membrane integrity were analyzed using the Kruskal-Wallis test. The results of data analysis showed that there were significant differences in motility, viability, and membrane integrity (P less than 0.05). The highest motility and viability with additional SBE treatment were 29,38 approximately 9,80 percent and 68,13 approximately 6,81 percent. The highest membrane integrity in the SBE + AR 1 percent + RO 1 percent + PO 1 percent treatment was 80.00 approximately 6.23 percent. The conclusion of this study was that the treatment with the addition of SBE only had the best effect on motility and viability, while the treatment with the addition of 1 percent AR, 1 percent ROS, and 1 percent PO had the best effect on membrane integrity.

Kata Kunci : Argan oil, Rosemary oil, Peppermint oil, Post-thawing, Direct swim up, Quality of Spermatozoa.