Persaingan Vigna radiata (L.) Wilezeck dan Cyperus rotundus L. pada lahan beragam dengan inokulasi rhizobium
NURHIDAYATI, Tutik, Prof.Dr. Santosa
2003 | Tesis | S2 BiologiKehadiran Cyperus rotundus (L.) di antara tanaman Vigna radiata (L.) dalam suatu ruang tumbuh yang terbatas menimbulkan persaingan. Pada lahan bergaram, persaingan semakin meningkat karena adanya cekaman salinitas. Tujuan penelitian ini untuk mengetahui persaingan Vigna radiata (L.) Wilezeck dan Cyperus rotundus (L.) pada lahan bergaram dengan inokulasi Rhizobium. Penelitian dilaksanakan menggunakan Rancangan Acak Lengkap Pola Faktorial dengan 5 ulangan. Faktor pertama jenis lahan yaitu : Penambahan garam NaCl 10 g / 5 kg tanah (N1) dan tanpa NaCl (N0). Faktor kedua inokulasi Rhizobium yaitu : dengan inokulasi Rhizobium (Pr) dan tanpa Rhizobium (P0). Faktor ketiga perbandingan jumlah Vigna radiata (L.) (Vr) dengan Cyperus rotundus (L.) (Cr) yang didasarkan pada replacement series yaitu : Vr/Cr meliputi rasio 0/4 (V0); 1/3 (V1); 2/2 (V2); 3/1 (V3); 4/0 (V4); serta kontrol untuk Vigna radiata 1/0 (K1); 2/0 (K2); 3/0 (K3). Analisis data menggunakan sidik ragam (ANAVA) dan uji lanjut dengan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukkan peningkatan jumlah Cyperus rotundus (L.) menekan pertumbuhan Vigna radiata baik pada lahan bergaram maupun pada lahan tidak bergaram. Pada kombinasi perlakuan 3/1 N1P0 dan 4/0 N1P0 pertumbuhan Vigna radiata lebih tinggi dibandingkan pertumbuhan Cyperus rotundus. Berat kering total tanaman Vigna radiata 4/0 N0Pr. Penambahan garam NaCl meningkatkan akumulasi prolin Cyperus rotundus dan Vigna radiata serta mengubah profil protein Vigna radiata (BM protein pada lahan bergaram 25.6 kDa dan BM protein pada lahan tidak bergaram 29.8 kDa). Peningkatan jumlah Cyperus rotundus menurunkan efektivitas simbiosis dan pertumbuhan Vigna radiata.
The existence of Cyperus rotundus (L.) among Vigna radiata (L.) in a limited growing space will create competition. In saline soil, competition increases since there is salinity stress. The aim of this study was to know the effects of Rhizobium inoculation on the competition of Vigna radiata (L.) Wilezeck and Cyperus rotundus (L.) in saline soil. Factorial Completely Randomized Design with 5 replicates was used in this study. The first factor, was the addition of NaCl 10 g/ 5kg soil (N1) and without NaCl (N0). The second factor, was Rhizobium inoculation : with Rhizobium inoculation (Pr) and without Rhizobium inoculation (P0). The third factor, was the mixing ratio of Vigna radiata (Vr) with Cyperus rotundus (Cr) based on replacement series. The ratio of Vr/Cr consisted of 0/4 (V0); 1/3 (V1); 2/2 (V2); 3/1 (V3); 4/0 (V4); as well as a control for Vigna radiata 1/0 (K1); 2/0 (K2); 3/0 (K3). The collected data were analyzed by using Standard Analysis of Variance (ANAVA) and advance test using Duncan’s Multiple Range Test (DMRT). The results of the study showed that in saline soil the increasing number of Cyperus rotundus more inhibited the growth of Vigna radiata. At the combination treatment of 3/1 N1P0 and 4/0 N1P0, the growth of Vigna radiata was higher than Cyperus rotundus. The highest Vigna radiata total dry weight was found in the combination treatment of 4/0 N0Pr. The addition of NaCl increased proline accumulation both in Vigna radiata and Cyperus rotundus. Vigna radiata protein molecular weight in saline soil was 25.6 kDa and 29.8 kDa in control soil respectively. The existence of Cyperus rotundus reduced both the effectivity of Rhizobium symbioses and the growth of Vigna radiata.
Kata Kunci : Biologi Tanaman,Pertumbuhan Tanaman Vigna Radiata,Inokulasi Rhizobium,Competition, replacement series, Rhizobium, proline, protein profile. plant growth
