PENGARUH PENAMBAHAN NANOFIBER SISAL (Agave Sisalana) BERBAGAI KONSENTRASI PADA SILER RESIN EPOKSI TERHADAP DAYA PEMBASAHAN DAN DAYA ANTIBAKTERI TERHADAP Streptococcus Mutans

FAUZIAH KARIMAH, Dr.drg Ema Mulyawati,MS.Sp.KG(K); Dr.drg. Tunjung Nugraheni, M.Kes.,Sp.KG(K)

2021 | Tesis-Spesialis | KONSERVASI GIGI

Penambahan suatu filler ke siler resin epoksi dapat meningkatkan sifat fisik dan sifat antibakterinya. Tujuan penelitian ini untuk mengetahui pengaruh penambahan nanofiber sisal berbagai konsentrasi pada siler resin epoksi terhadap daya pembasahan dan daya antibakteri terhadap Streptococcus mutans. Siler nanofiber sisal tiap kelompok konsentrasi 0%, 0,25%, 0,5%, 0,75%, 1% dan 1,25% dilakukan pengujian daya pembasahan dengan mengukur sudut kontak pada menit ke 3, hasil uji ditransfer ke image J modifikasi contact angle. Pengujian daya antibakteri siler nanofiber sisal dilakukan dengan metode difusi dan diinkubasi selama 48 jam pada suhu 37o, dan dihitung zona hambatnya. Hasil perhitungan dari uji daya pembasahan dan antibakteri dianalisis dengan Anava satu jalur. Hasil analisis Anava satu jalur menunjukkan sudut kontak <90o pada semua kelompok dan tidak terdapat perbedaan signifikan secara statistik (p>0,005) dan terdapat perbedaan yang signifikan secara statistik (p<0,005) pada uji antibakteri terhadap Streptococcus mutans. Kelompok nanofiber sisal 1% memiliki zona hambat paling tinggi, kedua adalah kelompok 0,5%, 0,75%, 1,25% yang memiliki daya hambat yang sama, ketiga kelompok 0,25% dan terendah kelompok 0% Kesimpulan penelitian ini adalah tidak terdapat pengaruh penambahan nanofiber sisal berbagai konsentrasi pada siler resin epoksi terhadap daya pembasahan dan terdapat pengaruh terhadap daya antibateri bakteri Streptococcus mutans.

The addition of a filler to the epoxy resin sealer can improve its physical properties and antibacterial properties. The purpose of this study was to determine the effect of adding sisal nanofibers of various concentrations to the epoxy resin sealer on the wettability and antibacterial properties of Streptococcus mutans. Sisal nanofiber sealers for each concentration group of 0%, 0.25%, 0.5%, 0.75%, 1% and 1.25% were tested for wettability by measuring the contact angle at 3 minutes. The antibacterial activity of the sisal nanofiber sealer was tested by diffusion method, and incubated for 48 hours at 37o, then the zone of inhibition was calculated. The calculation results from the wetting and antibacterial tests were analyzed with one-way Anova. The results of one-way ANOVA analysis showed a contact angle of <90o in all groups and there was no statistically significant difference (p>0.005) and there was a statistically significant difference (p<0.005) in the antibacterial test against Streptococcus mutans. Concentration of 1% had the highest inhibition zone, the second was 0.5%, 0.75%, 1.25% which had the same inhibitory power, and then 0.25% and 0% group was the lowest. The conclusion of this study is that there is no effect of adding sisal nanofibers of various concentrations to the epoxy resin sealer on the wettability and that there is an effect on the antibacterial properties of Streptococcus mutans bacteria.

Kata Kunci : sisal nanofiber, epoxy resin sealer, wettability, Streptococcus mutans.