IDENTIFIKASI DAN ANALISIS IN SILICO GEN PENYAN DI ACC DEAMINASE PADA Raoultella terrigena PCM8

SARASWATI, Dr. Ir. Ngadiman, M. Si. ; Muhammad Saifur Rohman, S.P., M.Si., M.Eng, Ph. D.

2021 | Tesis | MAGISTER BIOTEKNOLOGI

PGPB (Plant Growth Promoting Bacteria) penghasil Aminocyclopropane-1-Carboxylate Deaminase (ACCD) dapat dimanfaatkan untuk mengatasi hambatan pertumbuhan tanaman yang terpapar cekaman. Penelitian ini bertujuan untuk identifikasi dan analisis gen penyandi ACCD (acdS) pada bakteri Raoultella terrigana PCM8. Karakter strain PCM8 dikonfirmasi dengan uji morfologi, fisiologi, dan sekuen gen 16SrDNA. Identifikasi gen acdS pada strain PCM8 diperoleh dari hasil WGS (Whole Genomic Sequencing) dan karakterisasi ACCD dilakukan dengan analisis in silico. Hasil uji morfologi, fisiologi dan sekuen gen 16SrDNA memberikan pembenaran bahwa strain PCM8 adalah bakteri Raoultella terrigena. Berdasarkan anotasi WGS diketahui bahwa gen acdS strain PCM8 terletak pada lokus 19090 dalam DNA genom dan tersusun atas 978 nukleotida. Analisis in silico menunjukkan bahwa enzim ACCD strain PCM8 memiliki 325 asam amino, berat molekul 34,95 kDa, pI 5,06, dan residu katalitik berupa Lys51, Ser78, Tyr287, dan Thr288.

PGPB (Plant Growth Promoting Bacteria) producing Aminocyclopropane-1-Carboxylate Deaminase (ACCD) could be applied to overcome plant growth inhibition due to any stresses. This study aims to identify and analyze the ACCD encoding gene (acdS) in the bacterium, Raoultella terrigana PCM8. The character of the PCM8 strain was confirmed by morphological, physiological, and 16SrDNA gene sequencing tests. Identification of the acdS gene in the PCM8 strain was obtained from the results of WGS (Whole Genomic Sequencing) and the characterization of ACCD enzyme was carried out by in silico analysis. The results of the morphology, physiology and gene sequence of 16SrDNA confirmed that the PCM8 strain was the bacterium of Raoultella terrigena. Based on the WGS annotation, it was known that the acdS gene of the PCM8 strain is located at the locus 19090 of genomic DNA and contains 978 nucleotides. In silico analysis showed that the ACCD enzyme of PCM8 strain has 325 amino acids, molecular weight of 34.95 kDa, pI value of 5.06, and catalytic residues consist of Lys51, Ser78, Tyr287, and Thr288.

Kata Kunci : Raoultella terrigena, gen acdS, CC Deaminase, Whole Genome Sequencing, In Silico