ISOLASIDANUJISENSITIVITASEscherichiacoli TERHADAP ANTIBIOTIKAKANAMYCINDANNOVOBIOCINSUSU SAPIPERAHPENDERITAMASTITISSUBKLINIS DIKECAMATANPAKEM

SEPTIAN WIDIARTO, Dr.drh.Yanuartono,M.P.

2012 | Skripsi | S1 KEDOKTERAN HEWAN

Radangambingataumastitismerupakansalahsatupenyakit yangbanyak menimbulkankerugian.Penelitianinibertujuanuntukisolasi, identifikasidanuji sensitivitas bakteri Esherichia Coli penyebab mastitis subklinis terhadap antibiotikakanamycindannovobiocin.Sebanyak20sampelsususapidikulturpadamediaPlatAgarDarah(PAD)danMacConkeyAgar(MCA) kemudiandiinkubasi padasuhu 37ºCselama24jam. Koloniyangtumbuhdikelompokkansesuai denganmorfologi koloninyadandilanjutkanuji-uji biokimiadenganmenggunakanuji gula-gula, SulphideIndolMotility (SIM), Indol, TryptitaseSoyAgar(TSA) danSimonCitrate. Untuk mengetahuisensitivitas terhadapantibiotikaKanamycindanNovobiocinbakteri yangtelahdikulturpadamediaBrainHeartInfussion(BHI) kemudiandikultur pada media Muller Hinton Agar (MHA) dan diukur zona hambat setelah diinkubasipadasuhu37ºCselama24jam.Hasil penelitianmenunjukkanbahwadari 20sampel sususapidiperoleh11sampelterinfeksibakteriEscherichiacoli. Ujisensitivitasmenunjukkanbahwa7 isolat (63,6%) sensitif terhadap kanamycin, 4 isolat (36,3%) intermedietterhadap kanamycin, sedangkan untuk novobiocin 10 isolat (90,9%) resistenterhadapnovobiocindan1isolat(9,09%)sensitifterhadapnovobiocin.

Mastitisisoneofmanydiseasesthatcancauseanyharm. Thisresearchis aimedtoidentifyisolation,identificationandthesensitivitytest ofEscherichia colicauseofsubclinicalmastitisagainstkanamycinandnovobiocinantibiotics. Twentysamples ofcow’s milkareculturedonBloodplateagar(BPA) andMacConkeyAgar(MCA)andincubatedat 37oCfor24hours.Coloniesthat growaregroupedaccordingtotheir colonymorphologyandthencontinuedby biochemical test which are confectionerytest, SulphideIndol Motility (SIM), Indol, TryptitaseSoyAgar(TSA) danSimonCitrate. Toknowthesensitivityof bacteria toKanamycinandNovobiocinantibiotics, thebacteriathat havebeen culturedon BrainHeart Infussion (BHI), and thencultured againon Muller HintonAgar(MHA)aremeasuredofresistancezoneafterincubatedfor24hours on37oC.Theresults showedthat onceconfirmed,fromtwentysamples ofcow’s milk, obtain eleven samples that infected of Escherichia coli bacteria. The sensitivitytestshowedthatsevenisolate(63,6%)aresensitivetoKanamycin, four isolate(36,3%) areintermediate-sensitivetoKanamycin. WhileforNovobiocin, tenisolate(90,9%)areresistanceagainstit, andoneisolate(9,09%)aresensitive ofit.

Kata Kunci : Escherichiacoli,mastitis,subklinis,kanamycin,novobiocin