PROFIL BIOKIMIA DARAH SAPI PERA H YANG MENGALAMI KAWIN BERULANG

DHASIA RAMANDANI, Drh. Surya Agus Prihatno, M.P.

2011 | Skripsi | S1 KEDOKTERAN HEWAN

Kawin berulang pada sapi perah merupak an suatu gejala klinis yang menyebabkan gangguan reproduksi yang ditandai dengan panjangnya calving interval . Kekurangan kolesterol dapat mempengaruhi sintesis hormon steroid. Kekurangan kalsium menimbulkan retensi plasenta. Kekurangan glukosa menyebabkan terhambatnya perkembangan folikel dan kematian embrio. Kekurangan protein menyebabkan delayed ovulation. Tujuan dari penelitian ini adalah untuk mengetahui konsentrasi glukosa, total kolesterol, kalsium dan total protein pada sapi perah yang mengalami kawi n berulang. Penelitian ini menggunakan 10 ekor sapi perah peranakan Friesian Holstein yang mengalami kawin berulang. Pengambilan sampel darah dilakukan pada saat periode estrus dan dilakukan 3,5 jam sebelum sapi diberi pakan. Sebanyak 10 ml sampel darah dikoleksi melalui vena jugularis kemudian di lakukan analisis darah di LPPT UGM. Pemisahan serum dengan cara sentrifugasi dengan kecepatan 4000 rpm selama 10 menit atau 12000 rpm selama 2 menit. Analisis kadar total kolesterol, total protein, kalsium darah da n glukosa darah menggunakan Photometer Microlab 300 dengan metode analisa spektrofotometer. Hasil penelitian menunjukkan bahwa sapi perah yang mengalami kawin berulang, kadar glukosa darah, total kolesterol, total protein, dan kalsiumnya memiliki konsentrasi di bawah normal. Rata-rata konsentrasi glukosa darah 48.58 ± 6.675 mg/dl , total kolesterol 125.95 ± 38,108 mg/dl , total protein 6.815 ± 0.821 g/dl, dan kalsium darah 9.321 ± 0.94 mg/dl.

Repeated breeding in dairy cows is a clinical symptom that causes reproductive disorders are characterized by length of calving interval . Cholester ol deficiency can affect steroid hormone synthesis. Calcium deficiency causing retained placenta . Deficiency of glucose causes inhibition of follicular development and early embryo nic death . Protein deficiency causes delayed ovulation . The purpose of this study was to determine the concentration of glucose, total cholesterol, calcium and total protein in dairy cows that experience repeated breeding. This research used 10 Friesian Holstein dairy cows that experience repeated breeding . Blood samples were col lected during the period of estrus and taken 3.5 hours before the cows were fed . A total of 10 ml blood samples were collected via the jugular vein and blood analysis performed at LPPT UGM. Separation of serum by centrifugation at 4000 rpm for 10 minutes o r 12000 rpm for 2 minutes . Analysis of total cholesterol, total protein, blood calcium and blood glucose using a photometer Microlab 300 with a spectrophotometer analysis method . The results showed in dairy cows that experience repeated breeding, blood gl ucose, total cholesterol, total protein, and calcium has a concentrations below normal . The average concentrations blood glucose 6675 ± 48.58 mg / dl, total cholesterol 125.95 ± 38.108 mg / dl, total protein 6815 ± 0821 g / dl, and blood calcium 9321 ± 0.9 4 mg / dl.

Kata Kunci : Kawin berulang, Total kolesterol, Total protein, Kal sium, Glukosa