AKTIVITAS KITINASE Streptomyces sp. ISOLAT PB-2 DALAM MEDIUM KITIN YANG DITAMBAHKAN DENGAN BERBAGAI SUMBER NITROGEN

Virgiawan Fajar Nugroho, Indun Dewi Puspitas, S.P., M.Sc., Ph.D.

2021 | Skripsi | S1 TEKNOLOGI HASIL PERIKANAN

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan sumber nitrogen yaitu pepton, yeast extract, ammonium sulfat, dan ammonium klorida ke dalam medium kitin cair terhadap aktivitas kitinase Streptomyces sp. PB-2. Pengujian dilakukan pada suhu 30ºC dan pH 7 selama 7 hari masa inkubasi. Parameter yang diuji meliputi aktivitas kitinase (U/ml) dan konsentrasi N-asetilglukosamin (NAG) (mg/ml). Hasil penelitian menunjukan bahwa penambahan sumber nitrogen organik maupun anorganik secara signifikan (p<0,05) menurunkan aktivitas kitinase Streptomyces sp. PB-2 pada hari ke-1 sampai ke-5 inkubasi. Namun demikian pada hari ke-7 aktivitas kitinase pada perlakuan penambahan ammonium chloride cenderung meningkat dan menunjukkan hasil yang lebih tinggi dibandingkan control, dengan nilai sebesar 0,0021 U/ml. Penelitian ini menunjukkan bahwa penambahan sumber nitrogen berupa pepton, yeast extract, ammonium sulfat, dan ammonium klorida pada medium kitin cair tidak meningkatkan aktivitas kitinase Streptomyces sp. PB-2 hingga hari ke-5 inkubasi.

This study aimed to determine the effect of supplementing various nitrogen sources peptone, yeast extract, ammonium sulfate, ammonium chloride, into chitin broth medium on the chitinase activity of Streptomyces sp. PB-2. The examination was carried out at 30°C and pH 7 during 7 days incubation period, which is the optimum growth condition of Streptomyces sp. PB-2. Parameters examined included chitinase activity (U/mL) and concentration of N-acetylglucosamine (NAG) (mg/mL). The result showed that the supplementation of chitin broth with organic nitrogen and inorganic nitrogen significantly (p<0,05) reduced the chitinase activity of Streptomyces sp. PB-2 at the incubation period of day-1 to day-5. However at day-7 incubation, the trend of increasing in chitinase activity was observed for the treatment of ammonium chloride supplementation with the value of 0.0021 U/mL. This research showed that the supplementation of chitin broth medium with peptone, yeast extract, ammonium sulfate, and ammonium chloride did not increase chitinase activity of Streptomyces sp. PB-2 until 5 day incubation.

Kata Kunci : Aktivitas kitinase, ammonium klorida, ammonium sulfat, Streptomyces sp. PB-2, yeast extract