Pengaruh Lama Aplikasi Nanofiber Platelet-Rich Plasma Kitosan Polyvinyl Alcohol terhadap Kadar Transforming Growth Factor-Beta (Kajian High Performance Liquid Chromatography)

MEREZA PRAMUDYASARI, drg. Kwartarini Murdiastuti, Sp.Perio(K), Ph.D;drg. Vincensia Maria Karina, MDSc., Sp.Perio(K)

2021 | Skripsi | S1 KEDOKTERAN GIGI

Penggunaan nanofiber dalam terapi penyakit periodontal memudahkan proses regenerasi jaringan periodontal dengan prinsip Guided Tissue Regeneration (GTR). Freeze-dried platelet-rich plasma (FD-PRP) yang ditambahkan pada nanofiber dapat mempercepat regenerasi jaringan karena mengandung berbagai macam growth factor, termasuk Transforming Growth Factor-Beta (TGF-Beta) yang merupakan growth factor terbanyak dalam PRP. Adanya kitosan dan polyvinyl alcohol (PVA) yang ditambahkan pada nanofiber dapat mengendalikan kadar pelepasan TGF-Beta sehingga memberikan efek maksimal dalam proses penyembuhan penyakit periodontal. Penelitian ini bertujuan untuk mengetahui pengaruh lama aplikasi nanofiber PRP kitosan PVA terhadap kadar TGF-Beta. Pada penelitian ini, sampel yang digunakan adalah nanofiber PRP kitosan PVA yang diuji dengan High Performance Liquid Chromatography (HPLC). Sampel direndam dalam 10 ml larutan NaCl 0,9% untuk mengaktivasi TGF-Beta selama 15 menit, 60 menit, 1 hari, 3 hari, dan 10 hari dengan masing- masing kelompok waktu aplikasi yang terdiri dari lima sampel. Uji HPLC dilakukan untuk mengetahui kadar pelepasan TGF-Beta dalam lima waktu aplikasi tersebut. Data hasil uji HPLC dianalisis menggunakan uji ANAVA satu jalur dan uji korelasi Spearman. Hasil analisis statistik menunjukkan bahwa terdapat perbedaan signifikan rerata kadar TGF-Beta yang signifikan di antara kelompok (p<0,05) dan terdapat pengaruh lama aplikasi nanofiber PRP kitosan PVA terhadap kadar TGF-Beta (p>0,05) dengan korelasi yang sangat kuat antarvariabel (r=0,981). Adanya korelasi positif (+0,981) antarvariabel menunjukkan bahwa semakin lama waktu aplikasi, maka kadar TGF-Beta yang dilepaskan oleh nanofiber akan semakin banyak. Hal tersebut kemungkinan disebabkan oleh adanya penambahan kitosan pada nanofiber. Kesimpulan dari penelitian ini adalah lama aplikasi nanofiber PRP kitosan PVA berpengaruh terhadap kadar pelepasan TGF-Beta.

The periodontal tissue regeneration process can be facilitated by using nanofibers with Guided Tissue Regeneration (GTR) principle. Freeze-dried platelet-rich plasma (FD-PRP) is added in nanofibers to accelerate the tissue regeneration with the precense of growth factors, including Transforming Growth Factor-Beta (TGF-Beta) that is most abundant in amount. The release of TGF-Beta can be controlled by nanofiber with the addition of chitosan and polyvinyl alcohol (PVA), thus allowing it to give maximum effect in periodontal disease healing process. This study aims to find out the effect of the duration of PRP chitosan PVA nanofiber application on the level of TGF-Beta. In this study, PRP chitosan PVA nanofibers were used as samples for High Performance Liquid Chromatography (HPLC) test. Those samples were soaked in NaCl 0,9% solution to activate TGF-Beta within 15 minutes, 60 minutes, 1 day, 3 days, and 10 days with 5 samples for each application time group. HPLC test was conducted to get the release level of TGF-Beta during those five application times. The HPLC datas acquired were analyzed by using one way ANOVA test and Spearman correlation test. The statistical analysis result showed that there were significant mean differences between the groups (p<0,05) and there was an effect of nanofiber PRP chitosan PVA application time on the level of TGF-Beta (p>0,05) with a strong correlation between 2 variables (r=0,981). The positive correlation (+0,981) implied that the longer the time of application, the higher the level of TGF-Beta released from the nanofiber. It was possibly caused by the addition of chitosan to the nanofiber. The conclusion of this study is the duration of PRP chitosan PVA application gives an effect on the level of TGF-Beta.

Kata Kunci : nanofiber, platelet-rich plasma, kitosan, transforming growth factor-beta