EKSPRESI EKSOSOMAL URINE MRNA NCOA1 DAN MRNA MMP-9 TERKAIT METASTASIS KARSINOMA PROSTAT

NIMAS PRITA R K W, Indwiani Astuti; Nungki Anggorowati

2021 | Tesis | MAGISTER ILMU BIOMEDIK

NCOA1 dan MMP-9 memiliki peran dalam progresivitas dari karsinoma prostat. NCOA1 sebagai koaktivator yang berperan dalam membantu meningkatkan ekspresi gen biasaya memiliki konsentrasi rendah di sel normal. Adanya peningkatan aktivitas dari NCOA1 akan mengakibatkan peningkatan ekspresi gen target, salah satunya MMP-9. MMP-9 utamanya berperan dalam membantu invasi dan metastasis sel kanker. Penelitian ini bertujuan untuk mengkaji ekspresi mRNA NCOA1 dan MMP-9 dalam eksosom urine terhadap status metastasis pasien karsinoma prostat. Sampel berupa urine pasien karsinoma prostat Rumah Sakit Sardjito. Data berupa metastasis, Gleason score, kadar PSA, dan volume prostat diperoleh dari rekam medis. RT-PCR digunakan untuk menganalisis ekspresi NCOA1 dan MMP-9. Sebanyak 20 sampel dianalisis, 18 sampel memiliki data metastasis, 17 sampel memiliki data Gleason score dan kadar PSA, serta 12 sampel memiliki data volume prostat. Hasil analisis didapatkan tidak ada perbedaan ekspresi NCOA1 dan MMP-9 terhadap metastasis, Gleason score, dan kadar PSA, namun kadar MMP-9 didapatkan lebih tinggi pada prostat yang mengalami peningkatan volume. Tidak terdapat perbedaan ekspresi NCOA1 dan MMP-9 terkait metastasis karsinoma prostat.

NCOA1 and MMP-9 play an essential role in the development of prostate cancer. NCOA1 as a co-activator is a strong driver of gene expression. It tends to unstable and has a low concentration in normal cells. Deregulation of NCOA1 expression causes an increasing activity of NCOA1 that significantly affects the expression level of its target genes, including MMP-9. MMP-9 mainly has a role in assisting cancer cell invasion and tumor metastasis. This study aimed to elucidate the expression level of NCOA1 and MMP-9 in exosomes of urine in prostate cancer metastasis. The samples were urine of prostate cancer patient that diagnosed in Dr. Sardjito Hospital. Data of metastasis, Gleason score, PSA, and prostate volume were obtained from medical records. Reverse transcriptase-PCR (RT-PCR) was used to quantify and analyze NCOA1 and MMP-9 expressions. Twenty samples were analyzed, 18 had metastasis status, 17 had Gleason score, 17 had PSA level, and 12 had prostate volume data. The result showed that there was a difference in the expression level of MMP-9 in prostate volume but not in the expressions of NCOA1 and MMP-9 in metastasis, Gleason score, and PSA level. In conclusion, there was no difference between the expression of NCOA1 and MMP-9 in prostate cancer metastasis

Kata Kunci : NCOA1, MMP-9, eksosom, metastasis