PENGARUH SUPLEMENTASI ALMOND OIL DAN BLACK SEED OIL DALAM MEDIUM DIRECT SWIM UP SPERMA POST-THAWING TERHADAP KUALITAS SPERMATOZOA SAPI LIMOUSIN

KHAIRUNNISA S M, Widya Asmarawati, S.Pt., M.Sc.;Prof. Dr. Ir. Endang Baliarti, SU.

2021 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi Almond Oil (AO) dan Black Seed Oil (BSO) dalam medium direct swim up sperma post-thawing terhadap kualitas spermatozoa sapi Limousin. Penelitian dilakukan di Laboratorium Fisiologi dan Reproduksi Ternak Fakultas Peternakan Universitas Gadjah Mada. Materi penelitian yaitu 35 straw sperma beku sapi Limousin berumur antara 3 dan 4 tahun. Sperma beku di-thawing terlebih dahulu selama 30 detik pada suhu 37oC. Sperma post-thawing dicuci menggunakan metode direct swim up dengan medium Skim Base Extender (SBE) bersuplementasi AO atau BSO. Medium direct swim up terdiri dari tujuh perlakuan yaitu SBE (kontrol), SBE+AO 0,5%, SBE+AO 1%, SBE+AO 2%, SBE+BSO 0,5%, SBE+BSO 1%, SBE+BSO 2%. Data yang diperoleh meliputi motilitas, viabilitas, keutuhan tudung akrosom, integritas membran, dan abnormalitas tiap perlakuan dianalisis menggunakan uji Kruskal Wallis. Hasil analisis data menunjukkan bahwa penambahan suplementasi AO dan BSO terdapat perbedaan yang signifikan pada motilitas, keutuhan tudung akrosom dan integritas membran (P<0,05). Hasil motilitas dan tudung akrosom tertinggi pada perlakuan SBE+BSO 1% masing-masing 30,00+-9,13% dan 95,11+-1,30%. Integritas membran tertinggi dengan suplementasi AO 2% yaitu 83,14+-11,74%. Dari penelitian dapat disimpulkan bahwa penambahan suplementasi BSO 1% dalam medium direct swim up memiliki pengaruh paling baik terhadap motilitas dan keutuhan tudung akrosom spermatozoa, sedangkan suplementasi AO 2% paling baik terhadap integritas membran.

The research aimed to know the effect of almond oil and black seed oil supplementation in direct swim up medium post-thawing sperm on the quality of Limousin bull spermatozoa. The research was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, University of Gadjah Mada. The materials used in this study were 35 straws of Limousin bull frozen sperm between 3 and 4 years old. The frozen sperm were thawed for 30 seconds at 37oC. Post-thawing sperm were washed using the direct swim up method with the medium of Skim Base Extender (SBE) supplemented with Almond Oil (AO) or Black Seed Oil (BSO). The direct swim up medium consisted of seven treatments, that ware SBE (control), SBE+AO 0,5%, SBE+AO 1%, SBE+AO 2%, SBE+BSO 0,5%, SBE+BSO 1%, SBE+BSO 2%. Data observed were motility, viability, acrosome integrity, membrane integrity, and abnormality were analyzed using the Kruskal Wallis Test. The results of data analysis showed that the addition of AO and BSO supplementation were significantly different in motility, acrosomal integrity and membrane integrity (P<0,05). The highest motility and acrosome integrity was obtained in SBE+BSO 1% treatment was 30,00+-9,13% and 95,11+-1,30%. The highest membrane integrity with AO 2% supplementation was 83,14+-11,74%. From the result it concluded that the supplementation of BSO 1% in the direct swim up medium had the best effect on the motility and acrosomal integrity of spermatozoa, while AO 2% supplementation was the best for membrane integrity.

Kata Kunci : Almond Oil, Black Seed Oil, Direct Swim Up, Post-thawing, Quality of Spermatozoa.