Jumlah sel Leydig dan kadar testosteron mencit (Mus musculus L.) setelah perlakuan fotoperiode pendek

NUGROHO, Rudy Agung, Prof.Drh. M.P. Eddy Moeljono, MSA.,PhD.,SH

2002 | Tesis | S2 Biologi

Penelitian ini bertujuan mengetahui pengaruh fotoperiode pendek terhadap indeks berat testis, jumlah sel Leydig dan kadar testosteron mencit (Mus musculus L.). Hewan uji berupa dua puluh ekor mencit (Mus musculus L.) dipisahkan secara acak menjadi empat kelompok dengan jumlah masingmasing kelompok lima ekor hewan percobaan. Kelompok K sebagai kontrol dengan perlakuan fotoperiode dam; kelompok PI dengan fotoperiode 6T:18G; kelompok P2 dengan fotoperiode 3T:21G; dan kelompok P3 dengan fotoperiode OT:24G. Setiap kelompok ditempatkan dalam kandang polipropilen dan &masukkan dalam m g a n khusus kedap cahaya, kecuali kelompok K. Penyinaran (T) menggunakan Iampu fluoresense dengan intensitas 100-110 Lux diberikan setiap hari selama 35 hari &mulai tiap pukul 09.00 WIB. Selama perlakuan makan dan minum diberikan secara ad Zibzlm. Pada akhir perlakuan, hewan diambil darshnya dan serum dipisahkan untuk pengujian hormon testosteron. Hewan percobaan kemudian dikorbankan setelah sebelumnya ditimbang berat badan dan testis kanannya, untuk pengukuran indeks berat testis. Sed~aan histologi testis dibuat &engan metode parafin dan pewarnaan Hematoxylin-Eosin. Pengukuran kadar hormon testosteron menggunakan metode Radioimmunoassay (RIA). Hasil analisis indeks berat testis, jumlah sel Leydig dan kadar testosteron dianalisis dengan Anovu dan uji lanjut Duncan Multiple Range Test. Hasil penelitian menunjukkan bahwa fotoperiode pendek tidak mengakibatkan perbedaan signifikan terbdap indeks berat testis, namun akan menekan jumlah sel Leydig dan menurunkan kadar testosteron.

This research was conducted to assess the efect of short photoperiods on testicular weight index, number of Leydig cells and level of testosterone mice (Mus musculus L.). Twenty male mice were divided randomly into four groups, each consisted of Jive mice. Group K (Control) was treated with naturalphotoperiods; Groups PI with 6L (Lighr) : i8D (Dark) photoperiods; Groups P2 3L : 210 photoperiods; and Groups Pj OL : 240 photoperiods. Each group was placed in polypropylene cage and kept within light tight chamber, except for group K. Illumination (L) was given by cool white fluorescent lamp with light intensity of i00 la. Illumination was given for 35 days, every day and beginning at 09.00 (Greenwich Standard Time + 07.00 h). Food and water were available ad libitum At the end of the treatment, the blood was collected and the serum separated and jiozen for later testosterone analyses. AN animals were sacrlJiced and the right testis was removed to determine the testicular weight index and do the histological .(malyses. The right testis microanatomical preparations were made using parafin method, stained with Hematoxylin-Eosin to calculate the number of the Leydig cells. i?te measurement of the level of testosterone was done by using RIA (Radioimmuno Assay) method. The testicular weight index, the number of the Leydig cells, and the levels of testosterone were analyzed by using Anova and the Duncant Multipe Range Test (DMRT). The result showed that short photoperid were not significantly affect the testicular weight index, but reduced the number of the Leydig cells and levels of testosterone.

Kata Kunci : Mus musculus L., Fotoperiode, Indeks Berat Testis, Sel Leydig, Testosteron, Mus muculus L., Photoperiods, Testicular weight index, Leydig cells, Testosterone