Pengaruh implan progesteron intravagina dan Gonadotropin Releasing Hormone terhadap induksi birahi dan angka konsepsi sapi perah pasca beranak

WIDNYANA, I Gusti Ngurah Putu, Drh. Sri Hartantyo, MSc.,Ph.D

2002 | Tesis | S2 Sain Veteriner

Suatu penelitian telah dilakukan untuk mengetahui pengaruh implan progesteron intravagina dan gonadotropin releasing hormone terhadap induksi birahi dan angka konsepsi sapi perah pasca beranak. Tiga puluh dua (32) ekor sapi perah pasca beranak 60 hari atau lebih diseleksi dan dilakukan prakondisi sehingga score kondisi tubuh mencapai 3 - 4. Sapi - sapi tersebut dibagi secara acak menjadi empat kelompok dan selanjutnya akan mendapat perlakuan masing masing yakni perlakuan I ClDR 9 hari, perlakuan II ClDR 9 hari + GnRH, perlakuan 111 ClDR 15 hari dan perlakuan IV ClDR 15 hari + GnRH. GnRH diberikan saat inseminasi buatan dilakukan sementara inseminasi buatan dilakukan 58 dan 72 jam setelah implan dicabut. Data yang dikumpulkan meliputi data daya retensi implan ClDR dan angka deteksi estrus dianalisis secara diskriftif, data waktu timbulnya birahi dianalisis menggunakan rancangan pola faktorial 2 x 2, angka konsepsi dan angka NR 60 - 90 harilkebuntingan dianalisis menggunakan Chi - square. Sementara profil hormon progesteron dilakukan analisa menggunakan metode ELISA kit. Untuk mengetahui profil hormon progesteron dilakukan pengambilan sampel susu dari masing masing kelompok sebanyak dua ekor yakni pada hari ke 0, 5, 9/15, saat IB, hari ke 7, 11 dan 18 setelah inseminasi buatan. Hasil penelitian menunjukan bahwa retensi implan ClDR dan angka deteksi estrus 1 OO%, tidak menyebabkan radang pada vagina, waktu timbulnya birahi pada kelompok I (49,50 f 1,51) perlakuan II (49,25 k 1,67), perlakuan 111 (48,75 k 1,28) dan perlakuan IV (48,25 k 1,04, jam, setelah implan dicabut) berbeda tidak nyata (P>0,05). Angka konsepsi pada perlakuan I (62,5 %), perlakuan II (75,O %) perlakuan 111 (50,O %) perlakuan IV (62,5 %) berbeda tidak nyata (P>0,05). Angka NR 60 - 90 harilkebuntingan pada perlakuan I (62,5 %), perlakuan II (75,O %), perlakuan 111 (50,O %) dan perlakuan IV (62,5 %), berbeda tidak nyata (P>0,05). Namun demikian rata - rata konsentrasi progesteron susu pada hari ke 7, pada antara perlakuan II (CIDR 9 hari + GnRH) nyata lebih tinggi (Pc 0,05) dengan perlakuan I (CIDR 9 hari), 111 (CIDR 15 hari) dan 111 (CIDR15hari + GnRH) (13,16 k 3,43 vs 6,18 f 0,77 ; 8.72 f 2,47 ; 9,68 f 0,37 ng/ml), Sementara konsentrasi hormon progesterone hari ke 11 pada antara perlakuan I (CIDR 9 hari) dan perlakuan II (CIDR 9 + GnRH) nyata lebih tinggi (Pc 0,05) dengan perlakuan 111 (CIDR 15 hari) dan perlakuan II (CIDR 15 + GnRH) (15,30 k 1,19 dan 14,58 k 1,75 ng/ml vs 11,68 If: 335 dan 11,56 k 3,05 ng/ml).dan hari ke 18 pada perlakuan I (CIDR 9 hari) nyata leqh rendah (P< 0,05 dengan perlakuan II (CIDR 9 hari + GnRH) (16,44 rt 1,23 ng/ml vs 17,89 k 2,36 ng/ml).

The study was carried out to determine the effect of progesterone intravagina devices and gonadotropin releasing hormone on induction of estrus and conception rate in postpartum dairy cows. Thirty two postpartum dairy cows are selected and having body condition score 3 - 4 were used in this study. The cows were devided randomly into treatment I (CIDR 9 d), II (CIDR 9 d + GnRH), 111 (CIDR 15 d), and IV (CIDR 15 d + GnRH). The GnRH given at the time of insemination that was applied by fixed time 58 and 78 hour after progesterone device removal. These data likewise retention ClDR device, the effect of device on vagina, the estrous detection rate were analyzed descriptively, moreover induction of estrus were analyzed statistically using factorial 2 x 2. The conception rate , non return rate or pregnancy rate were tested by chi - Square. The milk sample were colleted at day 0, 5, 9/15, at the time of insemination, day 7, 11 and 18 after insemination. The profile progesterone concentration hormone were assayed by a commercial ELIZA kit. The result of this study showed that the retention ClDR devices and estrous induction was 1 OO%, there was no vagina irritation. Estrous induction recorded on treatment I, II, 111 and IV were 49.50 k 1.51 ; 49.25 rt 1.67 ; 48.75 rt 1.28 ; 48.25 f 1.04 hour, respectively. The conception rate data in treatment I, II, 111 and IV noted were 62.5 % ; 75.0 % ; 50.0 % ; 62.5 %, respectively. Also the NR 60 - 90 &pregnancy rate on treatment I, 11, 111, and IV noted were 62.5 % ; 75.0 % ; 50.0 % ; 62.5 % respectively. Unfortunately those data failed to show any significantly different (P>0.05). However, the mean milk progesterone concentration which take on day 7, the ClDR 9 day + GnRH treated cows had significantly higher (P< 0.05) compared to ClDR 9 days treated cows ; ClDR 15 day treated cows, and ClDR 15 day + GnRH treated cows (13.16 rt 3.43 vs 8.72 k 2.47 ; 6.18 f 0.77 and 9.68 f 0.37 nglml). The milk progesterone concentration which taken on day 11, the ClDR 9 treated cows and ClDR 9 + GnRH treated cows had significantly higher (P< 0.05) compared to ClDR 15 day treated cows and ClDR 15 + GnRH treated cows (15.30 k 1.19 and 14.58 f 1.75 ng/ml vs 11.68 k 3.55 and 11.56 rt 3.05 nglml). Moreover the milk progesterone concentration colleted on day 18, the ClDR 9 treated cows had significantly lower (PcO.05) compared ClDR 9 day + GnRH treated cows (16.44 f 1.23 ng/ml Vs 17.89 k 2.36 ng/ml).

Kata Kunci : lnduksi birahi, progesteron GnRH, angka konsepsi, inseminasi buatan, sapi perah pasca beranak, Estrus induction, progesterone, GnRH, conception rate, artificial insemination, postpartum dairy cows