FORMULASI MATRIKS PATCH TRANSDERMAL EKSTRAK DAUN TEH HIJAU (Camellia sinensis L.) Kuntze

EKA INDRA SETYAWAN, Prof.Dr. Akhmad Kharis Nugroho, M.Si.,Apt.

2020 | Disertasi | DOKTOR ILMU FARMASI

Camellia sinensis (teh hijau) memiliki banyak manfaat bagi kesehatan antara lain, antioksidan, antimutagenik, antikanker, antibakteri, antiobesitas, serta mampu mencegah hipertensi dan diabetes. Tujuan dari penelitian ini adalah membuat formula matriks patch transdermal ekstrak daun teh hijau (Camellia sinensis L.). Optimasi ekstraksi dilakukan dengan menggunakan metode faktorial desain sedangkan optimasi formula matriks patch transdermal dilakukan dengan metode simplex lattice design. Penetapan kadar asam galat, katekin, kafein, dan epigalokatekin galat (EGCG) dalam ekstrak daun teh hijau, pelepasan obat dan transpor membran dilakukan dengan menggunakan metode kromatografi cair kinerja tinggi (KCKT). Penentuan kinetika pelepasan obat ditentukan berdasarkan analisis fitting kurva menggunakan model orde nol, orde satu, Higuchi, dan Korsmeyer-Peppas. Kinetika transpor membran ditentukan dengan menggunakan pendekatan berbasis kompartemen. Kondisi ekstraksi optimal dihasilkan pada suhu air:jumlah penyeduhan:lama penyeduhan (95oC:1 kali:20 menit). Formula optimal diperoleh dengan kombinasi HPMC K100:HPMC K4M:PEG 400 (4,0:4,5:0,5). Kinetika pelepasan obat mengikuti model Korsmeyer-Peppas, sedangkan transpor membran dapat digambarkan dengan model tiga kompartemen

Camellia sinensis (green tea) has many health benefits, namely antioxidants, antimutagenic, anticancer, antibacterial, antiobesity, antihypertension and antidiabetic. This study was aimed to formulate a transdermal matrix patch using green tea leaf extract (Camellia sinensis L.). Extraction optimization was performed by the factorial design method while optimization of the transdermal matrix patch formula was carried out by the simplex lattice design method. Determination of gallic acid, catechin, caffeine, and epigallocatechin gallate levels in green tea leaf extract, drug release and membrane transport were carried out using the high-performance liquid chromatography method. Determination of drug release kinetics is based on curve fitting analysis using zero order, first order, Higuchi, and Korsmeyer Peppas models. Whereas the determination of membrane transport kinetics used a compartment-based approach. The results showed optimal extraction conducted at water temperature: brewing number: brewing time (95oC: 1 time: 20 minutes). While the optimal formula was obtained by a combination of HPMC K100: HPMC K4M: PEG 400 (4.0: 4.5: 0.5). The drug release kinetics followed the Korsmeyer - Peppas model, while membrane transport can be illustrated by a three compartment model

Kata Kunci : teh hijau, patch, eigalokatekin galat, optimasi