ISOLASI DAN IDENTIFIKASI SENYAWA AKTIF PADA KULIT KAYU MASSOIA (Crytocarya massoia) dan UJI AKTIVITAS SEBAGAI ANTIBAKTERI

ZUZAN CRYSTALIA GRIAPON, Dr. Deni Pranowo, M.Si; Dr. Tri Joko Rajarjo, M.Si

2020 | Skripsi | S1 KIMIA

Isolasi dan identifikasi senyawa aktif pada kulit kayu massoia (Crytocarya massoia) telah dilakukan. Isolasi kulit kayu massoia dilakukan menggunakan metode ekstraksi padat - cair (maserasi), ekstraksi cair-cair dan kromatografi kolom, selanjutnya identifikasi ekstrak kulit kayu massoia dilakukan menggunakan metode uji fitokimia, uji Gas Chromatograpy - Massa Spektrophotometry (GC-MS), dan uji 1H-NMR. Uji antibakteri dilakukan dengan metode difusi agar, terhadap bakteri Escherichia coli dan Staphylococcus aureus dengan variasi konsentrasi 250, 500, 1000 dan 1500 ppm. Hasil ekstraksi padat-cair memperoleh 6,1 g ekstrak kasar. Hasil ekstraksi cair-cair menggunakan pelarut n-heksana, etil asetat dan metanol diperoleh 1,8 g, 2,5 g, dan 1,8 g. Hasil uji fitokimia menunjukkan bahwa ekstrak kasar mengandung senyawa golongan alkaloid, terpenoid, tanin, dan flavonoid. Hasil uji GC-MS ekstrak mengandung senyawa 5,6-dihidro-6-pentil-2H-piran-2-non, 2-butilakrolein, isosianat sikloheksana, cis-2,7-nona diena, 2-etil-1,2,3,4-tetrahidro-naptalin dan 6-heptiltetrahidro-2H-piran-2-non. Hasil uji 1H-MNR fraksi 4 kolom menunjukkan sampel teridentifikasi 16 atom hidrogen. Hasil uji aktivitas sebagai antibakteri menunjukkan ekstrak n-heksana konsentrasi pada 1500 ppm, dikategorikan memiliki aktivitas penghambatan rendah, karena memiliki diameter zona hambat terhadap bakteri Escherichia coli dan Staphylococcus aureus sebesar 4,3 mm dan 4,1 mm.

Isolation and identification of active compounds of massoia (Crytocarya massoia) bark and it is antibacterial activity test was conducted. The isolation method used solid-liquid extraction, liquid-liquid extraction and column chromatograpy. It is a compound are identified using phytochemical test, gas chromatograpy-mass spectrophotometry (GC-MS), and 1H-nuclear magnetic resonance (1H-NMR). The antibacterial activity test was conducted by diffusion method, to Escherichia coli and Staphylococcus aureus bacterial in different concentration, were 250, 500, 1000 and 1500 ppm. The result of solid-liquid extraction was known 6.1 g crude. The result of Liquid-liquid extraction used hexane, ethyl acetat, and methanol solvent, were known 1.8 g, 2.5 g and 1.8 g. The result of phytochemical test were known alkaloids, terpenoids, tannin, and flavonoids. The compounds of massoia bark were known 5,6-dihydro-6-pentyl-2H-pyran-2-none, 2-butylactrolein, cyclohexane isocyanate, cis-2,7-nona-di-ena, 2-ethyl-1,2,3,4-tetrahydro-naptaline and 6-heptiltetrahidro-2H-piran-2-non. The result of column chromatograpy fraction 4 was known 5,6-dihydro-6-pentyl-2H-pyran-2-none. The fraction 4 was identified used spectrophotometry 1H-NMR, that sample has 16 hydrogen atoms. Antibacterial activity test was known hexane extract has antibacterial activity on 1500 ppm, then it was categorized as weak caused has inhibition zone to Escherichia coli and Staphylococcus aureus bacterial were 4 and 4.3. Key words: Bark of massoia, Solid-liquid extraction, Antibacterial.

Kata Kunci : Kulit kayu massoia, Ekstraksi padat-cair, Antibakteri.