Pengaruh Konsentrasi Ekstrak Kulit Kentang (Solanum tuberosum L.) terhadap Hidrofobisitas Bakteri Enterococcus faecalis ATCC 29212

AMANDA AYU AFIFAH K, drg. Ivan Arie Wahyudi, M. Kes., Ph. D; drg. Mayu Winnie Rachmawati, M. Sc., Ph. D; dr. Rini Maya Puspita, M. Sc.

2020 | Skripsi | S1 KEDOKTERAN GIGI

Enterococcus faecalis merupakan salah satu bakteri penyebab periodontitis apikal, bakteri ini banyak ditemukan di dalam permukaan saluran akar gigi dengan persentase 80-90%. Kulit kentang (Solanum tuberosum L.) mengandung zat aktif antimikroba, yaitu alfa-solanine, alfa-chaconine, komponen fenol (chlorogenic acid dan caffeic acid), serta flavonoid (quercetin). Komponen zat aktif ini diduga dapat menurunkan hidrofobisitas bakteri E. faecalis. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ekstrak kulit kentang (Solanum tuberosum L.) terhadap hidrofobisitas bakteri E. faecalis ATCC 29212. Hidrofobisitas bakteri E. faecalis diketahui melalui uji perlekatan bakteri terhadap heksadekana yang dibagi menjadi lima kelompok perlakuan, yaitu ekstrak kulit kentang 5%, 10%, 20%, kontrol negatif (akuades) dan kontrol positif (Chlorhexidine gluconate 0,12%). Indeks hidrofobisitas pada seluruh kelompok perlakuan diukur menggunakan spektrofotometer dengan panjang gelombang yang sama (lamda = 550 nm). Hasil uji statistik one-way ANOVA menunjukkan terdapat pengaruh konsentrasi ekstrak kulit kentang terhadap hidrofobisitas bakteri E. faecalis. Post-Hoc LSD menunjukkan terdapat perbedaan signifikan antara kelompok kontrol negatif dan seluruh kelompok perlakuan (ekstrak 5%, 10%, 20% dan kontrol positif) serta terdapat perbedaan signifikan antara kelompok ekstrak konsentrasi 10% dibandingkan dengan kelompok ekstrak 5% dan 20%. Pada penelitian ini dapat disimpulkan bahwa ekstrak kulit kentang konsentrasi 5%, 10%, dan 20% dapat menurunkan hidrofobisitas bakteri E. faecalis ATCC 29212.

Enterococcus faecalis is one of the main factors in causing apical periodontitis and is found to be the most abundant in the root canal surface with a percentage of 80-90%. Potato (Solanum tuberosum L.) peel is known for its antimicrobial active components such as alpha-solanine, alpha-chaconine, phenolic compounds (chlorogenic acid and caffeic acid), and flavonoids (quercetin). These active components can also reduce the hydrophobicity of the E. faecalis bacteria. The aim of this study was to determine the effects of potato (Solanum tuberosum L.) peel extract concentrations on the hydrophobicity of E. faecalis ATCC 29212. The hydrophobicity of E. faecalis was determined by using the bacterial adhesion test towards hexadecane and was divided into five treatment groups (5%, 10%, 20% of potato peel extract concentrations, a negative control (aquadest) and a positive control (0.12% Chlorhexidine gluconate)). Hydrophobicity index of each treatment groups were measured using a spectrophotometer at the same wavelength (lamda = 550 nm). One-way ANOVA showed an effect of potato peel extract concentrations to reduce E. faecalis bacteria hydrophobicity. Post-Hoc LSD test showed significant difference between negative control group compared with all treatment groups (5%, 10%, 20% extract concentrations and positive control). The 10% extract concentration had significant difference compared with 5% extract concentration and 20% extract concentration. In conclusion, potato peel 5%, 10%, and 20% extract concentrations are able to reduce the hydrophobicity of E. faecalis ATCC 29212.

Kata Kunci : ekstrak kulit kentang, hidrofobisitas, bakteri Enterococcus faecalis