ANALISIS FREKUENSI KARIOLISIS SEL EPITEL MUKOSA BUKAL PADA OPERATOR MESIN FOTOKOPI

FARETHA ARLISS NURHADWI, Dr. drg. Alma Linggar Jonarta, M.Kes.; drg. Tetiana Haniastuti, M.Kes, Ph.D.

2019 | Skripsi | S1 KEDOKTERAN GIGI

Operator mesin fotokopi selalu terpapar toner mesin fotokopi saat mengisi ulang dan memperbaiki mesin fotokopi. Toner mesin fotokopi mengandung zat-zat nanopartikel sitotoksik, misalnya karbon hitam dan polycyclic aromatic hydrocarbon (PAH) yang dapat meningkatkan frekuensi kematian sel epitel mukosa bukal. Kematian sel ditandai oleh terjadinya kariolisis yaitu larutnya kromatin akibat aktivitas DNase dan RNase. Tujuan penelitian ini adalah untuk mengetahui pengaruh paparan toner mesin fotokopi terhadap frekuensi kariolisis sel epitel mukosa bukal pada operator mesin fotokopi. Subjek penelitian terdiri atas 10 orang operator mesin fotokopi pada kelompok terpapar serta 10 orang pada kelompok kontrol. Sampel sel epitel mukosa bukal diambil dari rongga mulut menggunakan cytobrush, kemudian sampel diwarnai dengan metode pewarnaan Papanicolaou. Sampel diamati menggunakan mikroskop cahaya dengan perbesaran 400x untuk mendapatkan data frekuensi kariolisis perseratus sel. Data dianalisis menggunakan uji T independen (p<0,05). Hasil penelitian menunjukkan frekuensi kariolisis antara kelompok terpapar dan kelompok kontrol berbeda signifikan. Berdasarkan hasil analisis tersebut, dapat disimpulkan bahwa paparan toner mesin fotokopi meningkatkan frekuensi kariolisis sel epitel mukosa bukal.

Photocopier operator exposed to toner while reloading and fixing the machine. This toner composed of cytotoxic nanoparticles such as black carbon and polycyclic aromatic hydrocarbon (PAH) which might increase the frequency of buccal epithelial cell death. Karyolysis, the dissolution of chromatin, is a sign of cell death. The purpose of this research was to know the effect of toner exposure to the frequency of karyolysis on buccal epithelial cell of photocopier operator. The subject of this research consisted of 10 photocopier operators as the exposed group and 10 healthy people as the control group. The buccal epithelial cells were collected with a cytobrush, then stained by Papanicolaou staining method. The samples were observed under microscope with 400x magnification. Frequency of karyolysis was counted per 100 cells. The collected data was analysed using Independent-Samples T test (p<0.05). Result showed that the frequency of karyolysis between exposed group and control group were significantly different. It is concluded that the exposure of toner increase the frequency of karyolysis in the buccal epithelial.

Kata Kunci : kariolisis, operator mesin fotokopi, toner mesin fotokopi, sitologi eksfoliatif, karyolysis, photocopier operator, toner, exfoliative cytology