Kerusakan Ultrastruktur dan Penghambatan Pertumbuhan Miselium Ganoderma boninense oleh Senyawa Organik Volatil dari Nocardiopsis sp. GME01 dan GME22

MOHAMMAD RYAN A, Ir. Jaka Widada, Ph.D.; Prof. Ir. Triwibowo Yuwono, Ph.D.

2019 | Skripsi | S1 MIKROBIOLOGI PERTANIAN

Nocardiopsis sp. GME01 dan GME22 merupakan koleksi aktinomisetes Laboratorium Mikrobiologi Pertanian, Fakultas Pertanian, Universitas Gadjah Mada yang merupakan kontaminan pada kultur Streptomyces. Isolat tersebut diketahui mempunyai kemampuan menghambat Fusarium oxysporum melalui senyawa organik volatil (SOV). Meskipun demikian hingga saat ini SOV yang dihasilkan kedua bakteri tersebut belum teridentifikasi. Selain itu aktivitas antifungal SOV dari Nocardiopsis terhadap Ganoderma boninense belum diketahui. Penelitian ini bertujuan untuk mengevaluasi potensi Nocardiopsis sp. GME01 dan GME22 dalam menghambat Ganoderma boninense melalui SOV. Di dalam peneltian ini dilakukan uji aktivitas antifungal SOV Nocardiopsis sp. GME22 dan GME01 terhadap Ganoderma boninense menggunakan metode inverted petri dish dan identifikasi terhadap SOV kedua bakteri menggunakan metode SPME-GC- MS. SOV Nocardiopsis sp. GME22 dan GME01 mampu menghambat pertumbuhan Ganoderma boninense sebesar 62,6% dan 52,1% secara berturut turut. Analisis SPME- GC-MS mengungkap perbedaan profil SOV dari kedua Nocardiopsis yang berkontribusi pada perbedaan aktivitas penghambatan. Nocardiopsis sp. GME01 dan GME22 mampu menghambat pertumbuhan Ganoderma boninense melalui senyawa organik volatil.

Nocardiopsis sp. GME01 and GME22 are actinomycetes collection that were isolated as Streptomyces culture contaminant. Previous study revealed that Nocardiopsis sp. GME01 and GME22 were able to inhibit growth of Fusarium oxysporum through volatile organic compound (VOC). To date, however there has been no data of Nocardiopsis sp. GME01 and GME22 VOC's and on their specific antifungal properties against Ganoderma boninense. This study was conducted to evaluate the inhibitory action of Nocardiopsis sp. GME01 and GME22 against Ganoderma boninense. The antifungal activity of Nocardiopsis sp. GME01 and GME22 against Ganoderma boninense were determined by employing inverted petri dish method, and through the analysis of their volatile organic compounds (VOC) by using SPME-GC-MS. The result of this study demonstrated that Nocardiopsis sp. GME22 and GME01 VOC's were able to inhibit growth of Ganoderma boninense by 62,6% and 52,1% respectively. SPME-GC-MS analysis revealed differences of VOC profiles on both Nocardiopsis, which contributed to differences in inhibitory activity. Therefore, it is shown that Nocardiopsis sp. GME01 and GME22 were able to inhibit growth of Ganoderma boninense through volatile organic compounds.

Kata Kunci : Aktivitas Antifungal, Ganoderma boninense, Nocardiopsis, Senyawa Organik Volatil (SOV)