PENGARUH PENAMBAHAN CaC03 DAN LAMA PENYIMPANAN TERHADAP KUALITASCAMPURAN ISOLAT SELULOLITIK DAN APLIKASINYA SEBAGAI DONOR MIKROBIA PADA EVALUASI JERAMIPADISECARA IN VITRO

Purwanto, Dr. Ir. Zaenal Bachrudin, M.Sc

2000 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan CaC03 pada Isolat Campuran selulolitik unggul (ICSU) terhadap pH dan total koloni isolat selama penyimpanan, serta aplikasinya sumber mikrobia dalam uji kecemaan in vitro jerami padi. ICSU didapat dari koleksi laboratorium Biokimia Nutrisi, Jurusan Nutrisi dan Makanan Temak Fakultas Petemakan UGM. Isolat campuran umur 3 hari dimasukkan dalam botol penyimpan tertutup dengan penambahan CaC03 sebanyak 0,0%, 0,5%, 1,0%, dan 1,5% dengan volume masing-masing 30 ml. Sebelum botol ditutup, isolat dialiri gas N2 untuk memberikan suasana anaerobik dan selanjutnya disimpan pada suhu kamar. Pengambilan sampel dilakukan setiap 4 minggu sampai minggu ke-12, untuk diukur pH dan total koloni. Selanjutnya isolat dijadikan sebagai sumber mikrobia untuk mengukur kecemaan in vitro jerami padi. Jerami padi in vitro kemudian dianalisis kadar air dan abu sehingga diperoleh nilai kecemaan bahan kering (KCBK) dan nilai kecemaan bahan organik (KCBO). Data pH dan total koloni dianalisis dengan analisis variansi dengan rancangan split-splot, sedang data KcBK dan KcBO dianalisis dengan analisis variansi dengan rancangan acak lengkap pola searah. Perbedaan variabel kemudian diuji dengan Duncan’s New Multiple Range Test. Hasil analisis variansi menunjukkan bahwa lama penyimpanan dan kadar CaC03 memberikan pengaruh yang sangat nyata terhadap pH dan total koloni (P<0,01). Sedangkan analisis variansi terhadap KcBK dan KcBO memberikan pengaruh yang tidak berbeda nyata. Nilai pH tertinggi pada aras 1,0% dan berturut-turut 0,5%, 1,5% dan 0,0% .Nilai total koloni tertinggi pada aras 1,5% dan berturut-turut 1,0%, 0,5%, dan 0,0% .Nilai KcBK tertinggi pada aras 1,0% dan berturut-turut 0,0%, 1,5% dan 0,5% . Nilai KcBO tertinggi pada aras 1,0%dan berturut-turut 1,5%, 0,5%dan 0,0%. Kata kunci : Jerami Padi, Donor Mikrobia, Isolat Campuran Selulolitik, CaC03> , Kecemaan in vitro.

The research was conducted to investigate the influence of CaC03 suplementation in superior cellulolytic mix culture (SCMC) on pH and total colony, and the second objective of the experiment was the application of SCMC as microbial sources for analysis of in vitro feed degradation. The isolates used in this research were superior cellulolytic mix culture obtained from the stock culture collection of Nutritional Biochemistry Laboratory, Faculty of Animal Science, Gadjah Mada University. The 3 days old of the SCMC was storaged in the closed bottle, wich had been added with CaC03 at 0,0%; 0,5%; 1,0%; and 1,5% level. The SCMC as starter then was storage at room temperature in anaerobic condition. Anaerobic condition was achieved by gassing with N2 passed trough a needle into the bottle. Each bottle contents 30 ml of starter. The sample of SCMC were taken to be measured the pH and total colony every 4 weeks until 12th week. The analysis of rice straw digestibility by in vitro need the water and ask determination for obtaining the dry matter and organic matter digestibility value.The data of pH and total colony were analyzed using analysis of variance with a split-splot design. The data of dry matter and organic matter digestibility value analized using Completely Randomized Design one way classification. The different variable observed than were analysisi by Duncan’s New Multiple Range Test. The result of the research showed that pH and total colony starter were influenced (P < 0,01) by the CaC03 addition and storage periods. The kind of starter did not influence to dry matter and organic matter digestibility value. The higest pH was accurates at 1,0% CaC03 level, followed by 0,5%; 1,5% and 0,0% respectively. The higest total colony was found at 1,5 % CaC03 level, followed by 1,0 %; 0,5% and 0,0% respectively. The higest value of dry matter digestibility was found at 1,0% CaC03 level, followed by 0,0% ; 1,5% and 0,5% respectively. The higest value of organic matter digestibility was found at 1,0% CaC03 level, followed by 1,5 %; 0,5%and 0,0% respectively. Key words : Rice straw, Microbia sources, Cellulolitic mix-culture, CaC03> , in vitro analysis

Kata Kunci : Jerami Padi, Donor Mikrobia, Isolat Campuran Selulolitik, CaC03> , Kecemaan in vitro