PENGARUH PEMBERIAN PLATELET RICH PLASMA (PRP) TERHADAP KADAR TESTOSTERON DAN TUMOR NECROSIS FACTOR-ALPHA (TNF-alfa) INTRATESTIS PADA TIKUS YANG MENGALAMI STRES OKSIDATIF DIINDUKSI DENGAN D-GALAKTOSA

INTANIA RISKA PUTRIE, Dr. dr. Dicky Moch. Rizal, M.Kes., Sp.And., AIFM.; Dr. dr. Rul Afiyah Syarif, M.Kes

2019 | Tesis | MAGISTER ILMU BIOMEDIK

Latar Belakang: Stes oksidatif merupakan salah satu pemicu gangguan fertilitas pada pria. Peningkatan stres oksidatif memicu apoptosis disertai peningkatan TNF-alfa. Peningkatan kadar TNF-alfa yang berlebihan menurunkan kadar testosteron. Platelet rich plasma (PRP) berpotensi menurunkan kadar TNF-alfa dan meningkatkan kadar testosteron. Penggunaan PRP dalam penelitian dibidang reproduksi wanita memberikan hasil yang cukup baik, tetapi penggunaan PRP dalam bidang reproduksi pria belum banyak dilakukan. Tujuan Penelitian: Penelitian ini bertujuan untuk mengkaji pengaruh pemberian PRP terhadap kadar testosteron dan TNF-alfa intratestis tikus Wistar yang diinduksi D- galaktosa. Metode: Tikus Wistar jantan (3 bulan, 200-300gr, n=30) dibagi menjadi 5 kelompok: kelompok K (kontrol), P1 (D-galaktosa), P2 (D-galaktosa + 0,05 mL PRP), P3 (D- galaktosa + 0,1 mL PRP) dan P4 (D-galaktosa + 0,2 mL PRP). D-galaktosa diinjeksi setiap hari secara intraperitoneal dengan dosis 300 mg/kg berat badan dan PRP diinjeksikan 1 kali seminggu secara intratesticular selama 42 hari. Kadar testosteron dan TNF-alfa intratestis diukur menggunakan metode ELISA (Finetest). Hasil Penelitian: Berdasarkan penelitian diperoleh hasil bahwa kadar testosteron dan TNF-alfa intratestis menunjukkan terdapat perbedaan signifikan antara kelima kelompok (p<0,05). Kadar testosteron dan TNF-alfa intratestis kelompok perlakuan dengan PRP memiliki perbedaan signifikan dibanding kelompok tanpa PRP. Kadar testosteron intratestis tertinggi terdapat pada P4 (D-galaktosa + 0,2 mL PRP) dan kadar TNF-alfa intratestis terendah terdapat pada P2 (D-galaktosa + 0,05 mL PRP). Kesimpulan: Pemberian PRP menunjukkan hasil bahwa rerata kadar testosteron intratestis lebih tinggi, serta rerata kadar TNF-alfa intratestis lebih rendah dibandingkan dengan kelompok tanpa pemberian PRP pada testis tikus yang diinduksi D-galaktosa.

Background: Oxidative stress is one of triggers for male fertility problems. Increased oxidative stress stimulates apoptosis and increase TNF-alfa. Elevation in TNF-alfa levels reduce testosterone levels. Platelet rich plasma (PRP) which has many growth factors is claimed to reduce levels of TNF-alfa and increase testosterone levels. Research on PRP use in female reproductive showed good result, but research in male reproductive has not been conducted. Objective: To investigate the effect of PRP in intratesticular testosterone and TNF-alfa levels Wistar rat testes induced by D-galactose. Methods: Male Wistar rats (3 months, 200-300gr, n=30) were divided into 5 groups: K (Control), P1(D-galactose), P2 (D-galactose + 0.05 mL PRP), P3 (D -galactose + 0.1 mL PRP) and P4 (D-galactose + 0.2 mL PRP). D-galactose with dose 300 mg/kg body weight was injected daily via intraperitoneal route and PRP were injected once a week by intratesticular for 42 days. Intratesticular testosterone and TNF-alfa levels were measured with ELISA kit. Result: Based on this study, intratesticular testosterone and TNF-alfa levels showed significant differences between five groups (p<0.05). Intatesticular testosterone and TNF-alfa levels had significant differences between P1 group (D-galactose) and control. Treatment group with PRP (P2, P3, P4) had significant differences compare to P1 group (D-galactose). The highest intratesticular testosterone levels were found in D- galactose + 0.2 mL PRP and the lowest intratesticular TNF-alfa levels were found in D- galactose + 0.05 mL PRP. Conclusion: Administration of PRP on rats testes induced by D-galactose showed that intratestiscular testosterone levels was higher, and intratesticular TNF-alfa was lower than the group without PRP.

Kata Kunci : PRP, D-galaktosa, testis, kadar testosteron intratestis, kadar TNF-alfa intratestin, Platelet rich plasma, D-galactose, testes, intratesticular testosterone, intratesticular TNF-alfa