Optimasi Formula Lotion THPGV-0 dengan Kombinasi Asam Stearat dan Triethanolamine Menggunakan Metode Simplex Lattice Design

Rizky Amalia, Dr. Ritmaleni, S.Si.; Prof. Dr. Akhmad Kharis Nugroho, MSi., Apt.

2019 | Skripsi | S1 FARMASI

Tetrahidropentagamavunon-0 (THPGV-0) merupakan salah satu senyawa analog kurkumin yang diketahui memiliki aktivitas antioksidan yang lebih baik dari vitamin E. THPGV-0 mulai dikembangkan dalam berbagai bentuk sediaan salah satunya adalah lotion . Lotion THPGV-0 diformulasikan dengan kombinasi asam stearat dan triethanolamine sebagai emulgator. Tujuan penelitian ini untuk mengetahui komposisi asam stearat dan triethanolamine yang akan menghasilkan formula lotion THPGV-0 yang menghasilkan sifat fisik yang optimum. Penentuan formula optimum dengan metode Simplex Lattice Design menggunakan software Design Experts versi 10 dengan respon viskositas, adhesiveness, cohesiveness, dan pH. Stabilitas lotion akan diuji dengan metode dipercepat freeze and thaw dan sentrifugasi. Hasil uji formula optimum akan diverifikasi secara statistik dengan menggunakan metode One-Sample-t-Test dengan taraf kepercayaan 95%. Sifat fisik formula optimum akan dianalisis menggunakan One-Way ANOVA. Aktivitas antioksidan pada formula optimum akan diuji dengan metode DPPH. Hasil penelitian menunjukkan bahwa formula optimum lotion memiliki komposisi asam stearat 10,45% dan TEA 1,55%. Formula optimum lotion memiliki pH 7,709 dan viskositas 3405 mPa.S. Hasil uji stabilitas dipercepat menunjukkan lotion mengalami kenaikan viskositas dengan metode freeze thaw dan lotion menunjukkan hasil yang stabil dengan metode sentrifugasi. Pada uji antioksidan diketahui bahwa lotion memiliki sifat antioksidan dengan nilai IC50 112,093 mg/ml.

Tetrahydropentagamavunon-0 (THPGV-0) is one of curcumin analog that already known for its antioxidant activity. THPGV-0 has a better antioxidant activity than vitamin E. THPGV-0 has been made into several pharmaceutical forms such as lotion. THPGV-0 lotion been formulated with stearic acid and triethanolamine as emulgator. This study was aimed to determine the amount of stearic acid and triethanolamine as emulgator in order to obtain a lotion with a good physical quality and stable in storage. Optimization of THPGV-0 lotion formula was designed by using Simplex Lattice Design method. The stability of the lotion was tested by accelerated test with freeze thaw and sentrifugation method. The result of the optimum lotion test was statistically verified with one sample t-test. The result of lotion stability test was statistically analyzed with One-Way ANOVA. Analysis of radical scavenging activity was determined by DPPH method. The optimal lotion formula was achieved with 10.45% of stearic acid and 1.55% triethanolamine. The optimum lotion formula has pH value 7.709 and viscocity value 3405 mPa.S. Tests with freeze thaw test showing that lotion has increasing in viscosity and test with centrifugation indicate a stable lotion. The results showed that the THPGV-0 lotion has IC50 at 112,093 mg/ml.

Kata Kunci : THPGV-0, lotion, antioksidan, asam stearat, triethanolamine