MOLECULARLY IMPRINTED POLYMER CHITOSAN BERBASIS QUARTZ CRYSTAL MICROBALANCE UNTUK DETEKSI ALPHA PINENE

FADLUNISA, Dr. Eng. Kuwat Triyana, M.Si.

2019 | Skripsi | S1 FISIKA

Deteksi alpha Pinene penting untuk dilakukan karena alpha Pinene dapat menjadi indikator kesegaran tanaman dan kematangan buah. Paparan alpha Pinene dengan konsentrasi tinggi dapat mengganggu kesehatan. Oleh karena itu, fokus penelitian ini adalah pengembangan sensor alpha Pinene menggunakan Quartz Crystal Microbalance berlapis Molecularly Imprinted Polymer Chitosan. MIP Chitosan dan Non MIP Chitosan dilapiskan menggunakan metode spin coating dengan kecepatan pertama dan kedua sebesar 700 rpm selama 10 detik dan 1500 rpm selama 50 detik. Terbentuknya lapisan di atas QCM terbukti dengan selisih frekuensi lebih dari 1000 Hz sebelum dan sesudah pelapisan. Keberadaan template alpha Pinene terkonfirmasi dari munculnya ikatan CH3 pada lapisan MIP Chitosan sebelum pelepasan template. Template dilepas dengan dipanaskan pada suhu 130 derajat celsius selama 1 jam. Kenaikan frekuensi setelah pemanasan mengonfirmasi keberhasilan pelepasan template. Respon QCM MIP Chitosan lebih besar dibanding Non MIP Chitosan. Sensitivitas QCM MIP Chitosan lebih besar dibanding QCM Non MIP Chitosan dengan linearitas 0,999. LoD dan LoQ QCM MIP Chitosan lebih kecil dibanding QCM Non MIP Chitosan. QCM MIP Chitosan selektif terhadap senyawa gamma Terpinene, etanol, aseton dan benzene. Selektivitas ini menunjukkan terjadinya efek imprinting yang menyebabkan terjadinya mekanisme penangkapan molekul alpha Pinene secara spesifik oleh rongga matriks polimer serta adanya interaksi hidrogen antara keduanya.

Detection of Alpha Pinene is necessary because alpha Pinene is the indicator of herb freshness and fruit ripeness. Alpha Pinene exposure with high concentration can also affect body health. So, in this experiment, we developed alpha Pinene sensor based Quartz Crystal Microbalance coated by Molecularly Imprinted Polymer Chitosan. MIP Chitosan and Non MIP Chitosan were coated on QCM using spin coating method with the first and second speed is 700 rpm for 10 s and 1500 rpm for 50 s respectively. Forming of thin film on QCM was confirmed by getting frequency difference more than 1000 Hz before and after coating process. The existence of alpha Pinene was confirmed by the appearance of CH3 bond in MIP Chitosan spectrum before removing template. Template was removed by heating at 130 celcius degree for 1 hour. Frequency increasing after heating confirmed that template was successfully removed. QCM MIP Chitosan response was higher than QCM Non MIP Chitosan. Sensitivity of QCM MIP Chitosan has high sensitivity with linearity 0,999. LoD and LoQ of QCM MIP Chitosan is smaller than QCM Non MIP Chitosan. QCM MIP Chitosan has high selectivity cause its respon to gamma Terpinene, etanol, aseton and benzene compound is small. This selectivity shows that imprinting effect causes specific recognition mechanism of alpha Pinene molecule by cavity of matrix polymer and hydrogen interaction between them.

Kata Kunci : alpha Pinene, chitosan, MIP Chitosan, Non MIP Chitosan, QCM